Immunofluorescence Microscopy

Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which is conjugated with a fluorescent dye. Direct immunofluorescence staining has a primary antibody labeled with a fluorescence dye whereas indirect immunofluorescence staining has a secondary antibody labeled with a fluorochrome used to recognize a primary antibody.

Protocols » Molecular Imaging » Immunofluorescence Microscopy

Immunofluorescence Microscopy Protocol Links Sort by: Hits | Alphabetical

Blocking of unwanted non-specific staining in Immunofluorescence. - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/NonSpecificBlocking.html

Blocking of unwanted non-specific staining in Immunofluorescence. Blocking of endogenous enzymes, Blocking of endogenous fluorochromes, Blocking endogenous biotin , Blocking of endogenous Fc blocking, Blocking of crossreactive antigens in the tissue. Cattoretti. Columbia University - [Read Blocking of unwanted non-specific staining in Immunofluorescence.]

Cell Staining for Immunofluorescence Microscopy - http://www.bdbiosciences.com/pharmingen/protocols/Immunofluorescence_Microscopy.shtml

Cell Staining for Immunofluorescence Microscopy. Includes protocols for fixing the cells, Coverslip Preparation for Adherent Cells, Coverslip Preparation for Non-Adherent Cells, Paraformaldehyde Fixation, and Methanol/Acetone Fixation. Blocking protocols include blocking with primary antibody, and incubation with secondary antibody. - [Read Cell Staining for Immunofluorescence Microscopy]

Double immunofluorescence staining for BCL-6 - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/DoubleIFstain.html

Double immunofluorescence staining for BCL-6. unfixed or acetone-fixed specimens. dewaxed, antigen retrieved slides - [Read Double immunofluorescence staining for BCL-6]

Fluorescence Mounting Medium (Antifade) - http://spectorlab.cshl.edu/fluorescence_medium.html

Fluorescence Mounting Medium (Antifade). Spector Lab, CSHL. - [Read Fluorescence Mounting Medium (Antifade)]

Fluorescent Staining of Cells - http://ylwang.umassmed.edu/protocol/cfs/stain.htm

Fluorescent Staining of Cells protocol. Yu-li Wang Lab. Fluorescent Phalloidin Staining, Immunofluorescence Staining and procedures for staining. - [Read Fluorescent Staining of Cells]

Fluorochrome Absorption and Emission Spectra - http://www.bdbiosciences.com/pharmingen/protocols/Fluorochrome_Absorption.shtml

Fluorochrome Absorption and Emission Spectra. BD Biosciences. Includes spectra of Allophycocyanin (APC), BD Cy-Chrome, Fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), PE-Texas Redâ„¢, Peridinin chlorophyll protein (PerCP), PerCP-Cy5.5, APC-Cy7, Texas Redâ„¢. - [Read Fluorochrome Absorption and Emission Spectra]

General Immunofluorescence Protocol - http://www.bcm.edu/rosenlab/protocols/generalIF.pdf

General Immunofluorescence Protocol. Rosen Lab. Deparaffinize and rehydrate sections, Antigen retrieval methods, Proteinase K Antigen Retrieval, Urea Antigen Retrieval, Blocking buffers, - [Read General Immunofluorescence Protocol]

Immunofluorescence - http://www.kcl.ac.uk/depsta/biomedical/randall/methods/im-fluo.html

Immunofluorescence. Dr. Walter Steffen. Includes fixation and staining protocols. - [Read Immunofluorescence]

Immunofluorescence / confocal microscopy - http://depts.washington.edu/keck/susan.htm

B or T cells in suspension, adherent cells on chambered coverglass or chamberslides, cryostat sections of unfixed, OCT embedded tissue. Susan Anderson. - [Read Immunofluorescence / confocal microscopy]

Immunofluorescence Protocol - http://www.abgent.com/doc/if1

Immunofluorescence Protocol. ABGent. Includes tips and considerations for improving protocol. - [Read Immunofluorescence Protocol]

Immunofluorescence protocol - Formaldehyde fixation - http://www.immuquest.com/cexe/protocols.asp?ID=19

Immunofluorescence protocol - Formaldehyde fixation. Immunoquest. - [Read Immunofluorescence protocol - Formaldehyde fixation]

Immunofluorescence Protocols - http://www.science.mcmaster.ca/biochem/faculty/andrews/lab/projects/methodsandprograms/labman/tc19.html

Immunofluorescence Protocol. Andrews Lab. McMaster University. - [Read Immunofluorescence Protocols]

Immunofluorescence Reagents and Protocols - http://www.molbio1.princeton.edu/facility/flowcyt/if1.html

Immunofluorescence Reagents and Protocols. Staining Protocols. Princeton. - [Read Immunofluorescence Reagents and Protocols]

Immunofluorescence staining - http://www.graingerlab.org/Research/Protocols/MethodQIFstaining.html

Immunofluorescence staining. Includes Background, Procedure, Key points and materials / methods. Grainger Lab. - [Read Immunofluorescence staining]

Indirect immunofluorescence with preextraction (in situ cell fractionation) - http://www.natureprotocols.com/2006/12/05/indirect_immunofluorescence_wi.php

Indirect immunofluorescence with preextraction (in situ cell fractionation). Raffaella Di Micco and fabrizio d'Adda di fagagna. - [Read Indirect immunofluorescence with preextraction (in situ cell fractionation)]

Minimizing Background in Immunofluorescence Procedures - http://cyto.mednet.ucla.edu/Protocols/minimizi.htm

Minimizing Background in Immunofluorescence Procedures. Includes tips and methods on reducing background staining. Janis V. Giorgi Flow Cytometry Laboratory, UCLA. - [Read Minimizing Background in Immunofluorescence Procedures]

PR/BrdU double immunofluorescence - http://www.bcm.edu/rosenlab/protocols/PR_BrdU.pdf

PR/BrdU double immunofluorescence. Rosen Lab - [Read PR/BrdU double immunofluorescence]

Preparation of cytospin from single cell suspension - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/CytospinPreparation.html

Preparation of cytospin from single cell suspension. Columbia University. Cattoretti Lab. - [Read Preparation of cytospin from single cell suspension]

Preparing a cell block from cell lines / cell suspensions - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/Cell_block.html

Preparing a cell block from cell lines / cell suspensions. Cattoretti, Colombia University. - [Read Preparing a cell block from cell lines / cell suspensions]

Protocol for Immunofluorescence - http://www.bio.unc.edu/faculty/salmon/lab/protocolsimmunofluorescence.html

Protocol for Immunofluorescence - Salmon Lab. Making Boiled Donkey (or whatever) Serum (BDS) for Blocking. Making Heat-Inactivated Serum for Blocking. Mounting Media. - [Read Protocol for Immunofluorescence]

Staining for Surface Receptors - http://www.ucsf.edu/vonzast/feeding.html

Staining for Surface Receptors. Patricia Tsao, Von Zazst Lab. - [Read Staining for Surface Receptors]

Staining for total receptors - http://www.ucsf.edu/vonzast/permeab.html

Staining for total receptors. Patricia Tsao, Von Zastrow Lab. - [Read Staining for total receptors]

STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN - http://ylwang.umassmed.edu/protocol/cfs/dbp.htm

STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN. Indirect and direct methods. Yu li Wang labs. - [Read STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN]

Vital Staining of Chromosomes with Hoechst 33258 - http://ylwang.umassmed.edu/protocol/cfs/hoechst.htm

Vital Staining of Chromosomes with Hoechst 33258. Yu Li Wang labs. - [Read Vital Staining of Chromosomes with Hoechst 33258]

Yeast IF without Dehydration - http://genomics.princeton.edu/botstein/protocols/non_MeOH_IF.html

Yeast IF without Dehydration. Botstein Lab. Protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual. - [Read Yeast IF without Dehydration]