Mononuclear Cell Isolation Protocols

Protocols and information related to mononuclear isolation. Including: B Lymphocyte Isolation and T-Cell Isolation.

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Mononuclear Cell Isolation Protocols Categories

B Lymphocyte Isolation Protocols (5)

T-Cell Isolation Protocols (7)

Mononuclear Cell Isolation Protocols Protocol Links Sort by: Hits | Alphabetical

Enrichment of PBMCs with Monocytes Protocol - http://www.methods.info/Methods/Cell_biology/monocyte_enrichment.pdf

Protocol for enrichment of PBMCs with monocytes. The cell suspension obtained after this protocol contains 40-70% monocytes. This cell suspension is than used for positive or negative MACS separation. - [Read Enrichment of PBMCs with Monocytes Protocol]

Isolation and Culture of Mouse Bone Marrow-Derived Macrophages Protocol - http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=572&criteria=

Mononuclear phagocyte progenitor cells derived from femoral and tibial bone marrow are propagated in the presence of M-CSF. This macrophage growth factor is secreted by L929 cells and is used in the form of L929 cell conditioned medium. The progenitor cells proliferate and differentiate through monoblast, promonocyte and monocyte stages before maturing to macrophages. At this time the cells become firmly adherent to the culture vessel. - [Read Isolation and Culture of Mouse Bone Marrow-Derived Macrophages Protocol]

Isolation of Dendritic Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E66369ADF1DF1962F01D3DA073183AB&objectid=6674A1FCBE1A61EE40A5E3EE8FB368F5

Presents two methods for preparing dendritic cells (DCs), a highly specialized type of antigen-presenting cell (APC). The first method involves the isolation of DCs from mouse spleen, resulting in a cell population that is highly enriched in accessory cell and APC function. A support protocol for collagenase digestion of splenocyte suspensions is described to increase the yield of dendritic cells. The second method involves generating large numbers of DCs from mouse bone marrow progenitor cells. - [Read Isolation of Dendritic Cells Protocol]

Isolation of Monocytes from Enriched PBMCs using CD14 Magnetic Beads Protocol - http://www.methods.info/Methods/Cell_biology/Monocytes_positive.html

This protocol uses the PBMC fraction enriched in with monocytes by density gradient centrifugations (protocol may be found at www.methods.info). Reduction of the amount of microbeads in comparison to Miltenyi protocol reduces the costs of the experiment. - [Read Isolation of Monocytes from Enriched PBMCs using CD14 Magnetic Beads Protocol]

Isolation of Mouse Mononuclear Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E66362DD82ED3278430D1F3DE7BC6C7&objectid=66749068D6282E6462167D5CDE01FDFC

Covers techniques for preparing lymphoid cell suspensions and removing red blood cells and dead cells. - [Read Isolation of Mouse Mononuclear Cells Protocol]

Purification of Human Mononuclear Cells and Neutrophils Protocol - http://www.cbrinstitute.org/labs/springer/protocols/chunduk_mono_neutro.html

Protocol for the purification of human mononuclear cells and neutrophils. - [Read Purification of Human Mononuclear Cells and Neutrophils Protocol]

Purification of Monocytes Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./protocols.html~main

Protocol for the purification of monocytes. - [Read Purification of Monocytes Protocol]

Purification of Neutrophils Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./protocol1.html~main

Protocol for purification of neutrophils. - [Read Purification of Neutrophils Protocol]