Immunoassays

Immunoassays including western blot protocols, immunoblot, immunoprecipitation, chromatin ip (ChIP), and protein dot blot.

Protocols » Immunology » Immunoassays

Immunoassays Categories

@Western Blot Protocol (19)

@Immunoprecipitation Protocols (9)

@Chromatin Immunoprecipitation Protocols (20)

Immunoassays Protocol Links

Assay of Cytokines in Tissue Culture Supernatants Protocol The Bio-Plex cytokine assay employs a liquid suspension array for quantification of cytokines in tissue culture supernatants or serum. Using this 96-well microtiter plateformatted assay, it is possible to profile the level of multiple cytokines in a single well.

Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF Protocol Protocol for ELISA assay for NGF. Includes: ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUM; BLOCKING; SAMPLE PREPARATION; PREPARATION OF NGF STANDARDS; PROTEIN RECOVERY; DESIGNING THE PLATE; APPLYING THE STANDARDS AND SAMPLES; APPLYING THE MONOCLONAL; APPLYING SECONDARY ANTIBODY; APPLYING STREPTAVIDIN; CHROMAGEN DEVELOPMENT; READING THE PLATE.

IL-4 In Situ Staining Protocol Protocol for IL-4 in situ stainging.

In Vivo Cytokine Capture Assay (IVCCA) Protocol Protocol for in vivo cytokine capture assay (IVCCA).

Steroid Radioimmunoassay Protocol Protocol for steroid radioimmunoassay. Includes: SOLVENT DISTILLATION; PREPARATION OF PLASMA SAMPLES; EXTRACTION OF STEROIDS AND COLUMN PACKING; COLUMN CHROMATOGRAPHY; RADIOIMMUNOASSAY; SEPARATION OF BOUND AND FREE COUNTS; DIRECT ASSAYS; SHORT COLUMN CHROMATOGRAPHY.

Western Blot Analysis—Phosphoprotein-Specific Antibody Mixture Protocol 3-step process of Western immunoblotting for detection of particular sites of phosphorylation on specific proteins recognized by modification-sensitive antibodies.