Immunology

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Immunocytochemistry Protocols (10)

Immunofluorescence Protocols (10)

Immunoprecipitation Protocols (13)

Macrophage Monocyte Protocols (2)

Mononuclear Cell Isolation Protocols (10)

T Cell Protocols (14)

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Epstein-Barr Virus Transformation of Lymphoblasts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4481

Protocol for Epstein-Barr Virus transformation of lymphoblasts. Protocol describes a method for the transformation of lymphoblasts by Epstein-Barr Virus (EBV). Cells may be isolated from whole blood or taken from cryopreserved, non-immortalized stocks. - [Read Epstein-Barr Virus Transformation of Lymphoblasts Protocol]

Preparation Of Peptide-KLH Conjugates For Immunization - http://mitchison.med.harvard.edu/protocols/ab1.html

Checking Peptide Thiol Groups, Coupling of Peptide to KLH. Claire Walczak - [Read Preparation Of Peptide-KLH Conjugates For Immunization]

Testing Rabbit Bleeds By Elisa - http://mitchison.med.harvard.edu/protocols/ab2.html

Coupling Peptide to BSA, ELISA with Peptide conjugated to BSA. Claire Walczak - [Read Testing Rabbit Bleeds By Elisa]

Phycoerythrin Conjugation Protocol - http://pingu.salk.edu/flow/protocols/pelabel.html

Protocol for phycoerythrin conjugation. Includes: Preparation of PE; SPDP modification of PE; SMCC modification of antibody; DTT treatment of SPDP-PE; Purification of reactants; Conjugation; Stop reaction; Concentrate product; Separate conjugate. - [Read Phycoerythrin Conjugation Protocol]

Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/Hematopoietic.pdf

Protocol for purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion. Includes: Harvest Marrow; Lineage Depletion; Cell Sorting; Viral Transduction; Transplant; CFU assays; GFP and cell surface marker immunostaining. - [Read Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells Protocol]

Minimizing Background in Immunofluorescence Procedures - http://cyto.mednet.ucla.edu/Protocols/minimizi.htm

Minimizing background in immunofluorescence procedures. - [Read Minimizing Background in Immunofluorescence Procedures]

RBC Lyses Solutions and Cell Lyses Protocol - http://depts.washington.edu/immunweb/cellanalysis/RBClyses.htm

Protocol for RBC lyses solutions and Cell lyses. - [Read RBC Lyses Solutions and Cell Lyses Protocol]

Detection of Autoantibodies with Self-Assembling Radiolabeled Antigen Tetramers Protocol - http://www.natureprotocols.com/2007/01/29/detection_of_autoantibodies_wi.php

Protocol for detection of autoantibodies with self-assembling radiolabeled antigen tetramers. Details how to produce radiolabeled antigen-streptavidin tetramers for detection of antibodies by immunoprecipitation. Optionally, the antigen tetramers can be denatured to compare responses to folded and unfolded antigen in the same system. This technique can be applied to a large or small number of samples, and a given sample can be simultaneously assayed with multiple antigens. - [Read Detection of Autoantibodies with Self-Assembling Radiolabeled Antigen Tetramers Protocol]

Method for Ameliorating Autoimmune Disease by Passive Transfer of IVIg-Primed Leukocytes - http://www.natureprotocols.com/2006/06/30/a_method_for_ameliorating_auto.php

A method for ameliorating autoimmune disease by passive transfer of IVIg-primed leukocytes. Developed a method for ‘priming’ specific leukocyte populations with IVIg to study their potential role in ameliorating ITP upon passive transfer, which we describe herein. Using this technique, found that splenic CD11c+ dendritic cells reacted with IVIg, or several IVIg mimetic regimes, were primed to ameliorate ITP upon transfer to thrombocytopenic mice. - [Read Method for Ameliorating Autoimmune Disease by Passive Transfer of IVIg-Primed Leukocytes]

Removal of CCR5 Ligands and Induction of Pro-Resolving Lipid Mediators by Apoptotic Neutrophils - http://www.natureprotocols.com/2006/10/02/removal_of_ccr5_ligands_and_in.php

Removal of CCR5 ligands and induction of pro-resolving lipid mediators by apoptotic neutrophils during resolution. Application of lipid extraction from peritoneal exudates, in tandem with lipid mediator informatics can be used to determine the role of apoptotic neutrophils in the generation of resolution phase lipid mediators. This neutrophil transfer system allows the determination of the direct impact of apoptotic leukocytes in the resolution of inflammation. - [Read Removal of CCR5 Ligands and Induction of Pro-Resolving Lipid Mediators by Apoptotic Neutrophils]