PCR Genotyping

Protocols and information related to PCR genotyping.

Protocols » Genetics and Genomics » Genotyping Protocols » PCR Genotyping

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Genotyping Embryonic Stem (ES) Cell Colonies Prior to Picking Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4415

This protocol describes a rapid PCR-based method for identifying targeted ES cell colonies prior to picking. It is based on DNA analysis of a small part of colonies pooled directly from selection plates. Only positive colonies are expanded. - [Read Genotyping Embryonic Stem (ES) Cell Colonies Prior to Picking Protocol]

Haplotype-Fusion PCR Protocol - http://www.natureprotocols.com/2006/07/10/haplotypefusion_pcr.php

Developed PCR-based single molecule haplotyping methods that enable both surveys for novel inversion variants, and population-scale genotyping of known inversions - [Read Haplotype-Fusion PCR Protocol]

PCR Genotyping from Tail DNA Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/P4.html

Protocol for PCR genotyping from tail DNA. This protocol works well for a variety of genes and primer pairs including Tg and KO alleles. Oligonucleotide melting temperatures between 60° and 65° seem to work well. - [Read PCR Genotyping from Tail DNA Protocol]

PCR Genotyping Primer Pairs Protocols - http://mgc.wustl.edu/protocols/pcr_primer_set.html

Primer pairs will amplify sequences present as a single copy in the mouse genome with the Universal Genotyping Protocol. Includes: b-Galactosidase (LacZ); cre-recombinase; CFP; diphtheria toxin; dsRED; Fabpi-200; Fabpi-500; flp recombinase; GFP/BFP/YFP; human growth hormone (complete); human growth hormone (transcriptional stop); luciferase (click-beetle); luciferase (firefly); neomycin phosphotransferase; SRY (male-specific); tTA (tet-on). - [Read PCR Genotyping Primer Pairs Protocols]

PCR Genotyping Protocol - http://www.duke.edu/~asl10/pcr_genotyping_protocol.htm

Protocol for PCR genotyping. - [Read PCR Genotyping Protocol]

PCR-Based Technologies Amplified Fragment Length Polymorphisms (AFLPs) - http://www.igd.cornell.edu/MolecularMarkers/AFLPs.pdf

Using molecular marker technology in studies on plant genetic diversity. DNA-based technologies: PCR-based technologies Amplified fragment length polymorphisms (AFLPs. Includes: AFLP technology, step by step; DNA digestion and ligation; PCRs and detection; Summarising the technology. - [Read PCR-Based Technologies Amplified Fragment Length Polymorphisms (AFLPs)]

Practical PCR Genotyping Protocols for Plasmodium vivax using Pvcs and Pvmsp1 - http://bmc.ub.uni-potsdam.de/1475-2875-4-20/

Plasmodium vivax is the second most prevalent malaria parasite affecting more than 75 million people each year, mostly in South America and Asia. In addition to major morbidity this parasite is associated with relapses and a reduction in birthweight. Practical PCR genotyping protocols based on polymorphic loci present in two P. vivax genetic markers. - [Read Practical PCR Genotyping Protocols for Plasmodium vivax using Pvcs and Pvmsp1]

Universal Mouse Genotyping Protocol - http://mgc.wustl.edu/protocols/pcr_genotyping.html

This protocol is designed to detect sequences in the murine genome by polymerase chain reaction amplification, and is adapted from Stratman and Simon (Transgenic Res. 12, 521-522 (2003)).For those familiar with PCR genotyping, this method differs from the typical protocol by utilizing a unique enzyme (Klentaq), 30mer primers, and a 68° annealing temperature. - [Read Universal Mouse Genotyping Protocol]