Single Strand Conformational Polymorphism(SSCP) Protocols

Detection of Mutations by Single-strand Conformational Polymorphism Protocol Single-strand conformational polymorphism (SSCP), one of several methods used to scan segments of DNA for mutations, exploits the electrophoretic differences in mobilities between single-stranded mutant and wild-type DNAs.

Mutation Detection By Single-Strand Conformational Polymorphism (SSCP) Protocol Protocol for mutation detection by single-strand conformational polymorphism (SSCP). Includes: PCR Protocol; SSCP Gels.

Single-Strand Conformation Polymorphism Analysis Protocol Single-strand confirmation polymorphism analysis (SSCP) is a powerful and robust method for the detection of DNA sequence changes (single-base substitutions) based on shifts in electrophoretic mobility. In this protocol, the target sequence is simultaneously labeled and amplified, then heat-denatured and resolved by non-denaturing polyacrylamide gel electrophoresis.