DNA Microarray Protocols

Hegde et al., Array Fabrication, microarray Printing, Probe Preparation and Hybridization, RNA Extraction, RNA labeling, Data Collection, Normalization, and Analysis.Institute for Genomic Research, Rockville, MD - [Read A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis]

Micro Array Fabrication, Probe Preparation and Hybridization, and Data Collection, Normalization and Analysis. Hegde, et al. biotechniques 2000. - [Read A Concise Guide to cDNA Microarray Analysis – II PDF.]

This protocol describes the labeling of eukaryotic RNA with aminoallyl labeled nucleotides via first strand cDNA synthesis followed by a coupling of the aminoallyl groups to either Cyanine 3 or 5 (Cy 3/Cy5) fluorescent molecules. Hasseman. TIGR Microarr - [Read Amino-allyl Labeling]

Chromatin Immunoprecipitation Protocol for Microarray Analysis – Protein A/G Bead Method. Protocol From the UHN Microarray Centre - [Read Chromatin Immunoprecipitation Protocol for Microarray Analysis]

Reagents & Equipment, Labelling, Purification, Hybridisation, Washing. KRL Kidney Research Dr.Healt - [Read Complete Microarray Chip Protocol]

Cyanine Dye Purification Protocol using Sep-Pak and HPLC purification protocol for Cy dyes (un)incorporated with dNTP. Brown Lab Howard Hughes Medical Institute - [Read Cyanine Dye Purification Protocol Cy]

Protocols, Frequently Asked Questions, Validation. Dr. Bart Frank - [Read DNA Microarray Procedures]

This protocol describes the steps required to produce a cDNA microarray. Gene-specific DNA is produced by PCR amplification of purified template plasmid DNAs from cloned ESTs. The PCR product is purified by ethanol precipitation, thoroughly resuspended in - [Read Fabrication Protocol for DNA Microarrays]

Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation Washing and Scanning Arrays. Brown Lab. - [Read Human DNA Microarray Hybridization]

Hybridization of Arrays. . General/Yeast Microarrays. Brown Lab - [Read Hybridization of Arrays]

Hybridization Protocol for Hybridizing fluorescent cDNA to slide. - [Read Hybridization Protocol for Hybridizing fluorescent cDNA to slide]

This protocol describes the hybridization of a Cy labeled cDNA probe (mix of Cy3 and Cy5) onto coated slide spotted with PCR amplified cDNA. TIGR Microarray Protocols. - [Read Labeled Probe Hybridization Procedure]

List of DNA Microarray Protocols. microarrays.org - [Read List of DNA Microarray Protocols]

Making a scanning array: in situ synthesis of oligonucleotides on a solid substrate. Elder et al., Oxford Practical Approach Series. - [Read Making a scanning array: in situ synthesis of oligonucleotides on a solid substrate]

This protocol describes the method for making microarray printing plates in a 96 well format. (The same procedure applies to a 384 well format as well.) Hasseman. TIGR Microarray Protocols - [Read MAKING MICROARRAY PRINTING PLATES (IN DMSO)]

This protocol describes clone handling, plate replication, and DNA template preparation in a 96 well format. Hasseman. TIGR Microarray Protocols - [Read MICROARRAY cDNA CLONE GROWTH AND TEMPLATE]

This protocol describes PCR amplification of eukaryotic cDNA plasmid inserts, gel electrophoresis, purification, and storage of PCR products. Hasseman. TIGR Microarray Protocols. - [Read MICROARRAY PCR, PURIFICATION, AND STORAGE]

Heather R. Sanders. Hybridization, Probe Creation and RNA preparation for Microarray Protocols. - [Read Microarray Protocols]

The "7 keys to successful microarray analysis" has been designed to guide researchers step-by-step through the design, implementation, analysis, and publication of a microarray experiment. - [Read Microarray Success]

Microarray fabrication, coating, slide blocking, printing microarrays, RNA extraction, Sample Labeling for analysis, hybridization, complete protocol. NHGRI - [Read Microarrays a Complete Manual PDF]

Post-Processing of Arrays. Brown Lab. - [Read Post-Processing of Arrays]

Reverse transcriptase labeling with Cy3/Cy5, Staining of total DNA on slides, construction, Laser scanning, Spot quantitation, Statistics, sample tracking. Vodkin Laboratory. U.Illinois. - [Read Protocols for microarray development in use in the Vodkin Laboratory]

Reverse transcription labeling using Cy3 and Cy5. Sample Labeling for Microarray Analysis. NHGRI - [Read Sample Labeling for Microarray Analysis]

Reusing DNA Microarrays using Stripping. Elder et al Oxford Practical Series. - [Read Stripping DNA Microarray array for re-use reusing]

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

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Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

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Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

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