Dideoxy Mediated Sequencing Protocols

Dideoxy-mediated Sequencing of DNA Using Taq DNA Polymerase Protocol Because sequencing reactions catalyzed by thermostable DNA polymerases such as Taq are carried out at elevated temperatures, problems caused by mismatched annealing of primers or templates rich in secondary structure are greatly alleviated.

Dideoxy-mediated Sequencing Reactions Using Bacteriophage T7 DNA Polymerase (Sequenase) Protocol Protocol describes DNA-sequencing of single-stranded DNA templates in reactions catalyzed by Sequenase.

Dideoxy-mediated Sequencing Reactions Using PCR and End-labeled Primers Protocol In this protocol asymmetric PCR is used to generate single-stranded DNA templates for dideoxy-sequencing

Dideoxy-mediated Sequencing Reactions Using the Klenow Fragment of E. coli DNA Polymerase Protocol for dideoxy-mediated sequencing reactions using the Klenow fragment of E. coli DNA polymerase I and single-stranded DNA templates.

Preparing Denatured Templates for Sequencing by Dideoxy-mediated Chain Termination Protocol Protocol describes how double-stranded plasmid DNA is denatured by alkali and annealed to an appropriate oligonucleotide primer, in preparation for dideoxy-sequencing reactions catalyzed by Sequenase.