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Cloning PCR products using TOPO cloning vectors. TOPO TA vectors from invitrogen. Protocols and methods for TOPO ligations and cloning.
| Simple TOPO Cloning Protocol - http://links.baruch.sc.edu/scael/notebook/cloning_protocol.doc TOPO cloning protocol. Farmer Lab. Tips and protocol for TOPO cloning from fresh PCR. - [Read Simple TOPO Cloning Protocol] |
| Topo Cloning Procedure - http://preuss.bsd.uchicago.edu/protocols/topo.html Topo Cloning procedure for cloning of small amounts DNA fragments by PCR. Cloning the PCR fragment into the TOPO vector, transforming E. Coli cells, and using blue/white selection to determine transformants. Culture inoculation, Qiagen Plasmid Prep protocol for plasmid isolation of the plasmid containing the cloned copies. Preuss Lab, Univ. Chicago. - [Read Topo Cloning Procedure] |
| TOPO Cloning Simple Protocol PDF - http://www.colorado.edu/MCDB/odorizzilab/TOPOcloning.pdf TOPO Cloning Simple Protocol. PDF Odorizzi Lab. Univ. Colorado. - [Read TOPO Cloning Simple Protocol PDF] |
| TOPO invitrogen Protocol - http://www.plantbio.uga.edu/~kelly/courseInfo/3210/ligation_transformation.pdf TOPO invitrogen Protocol - [Read TOPO invitrogen Protocol] |
| Zero Blunt TOPO PCR Cloning Kit Protocol - http://www.hawaii.edu/microbiology/MO/topo.htm Zero Blunt TOPO PCR Cloning Kit, Detailed protocol. (Invitrogen Life technologies). Alam Lab. Department of Microbiology, University of Hawai'i. - [Read Zero Blunt TOPO PCR Cloning Kit Protocol] |