SNP Protocols

FP-TDI Method for SNP Detection. The FP-TDI protocol was originally reported by Drs. Chen, Levine, and Kwok at Washington University in 19991,2. FP-TDI stands for template directed dye terminator incorporation assay with detection by fluorescence polarization. It is a single base primer extension assay couple with homogeneous FP detection. Perkin Elmer - [Read FP-TDI Method for SNP Detection]

High-resolution SNP mapping by denaturing HPLC. A SNP mapping procedure that relies on resolving polymorphisms by denaturing HPLC without the necessity of determining the nature of the SNPs. They demonstrate the use of denaturing high-performance liquid chromatography to identify mutations in the candidate genes and to fine-map chromosomal breakpoints. - [Read High-resolution SNP mapping by denaturing HPLC]

MULTIPLEX SNP ANALYSIS: SCREENING FACTOR V R506Q (LEIDEN) MUTATIONS. Beckman Coulter. - [Read MULTIPLEX SNP ANALYSIS: SCREENING FACTOR V R506Q (LEIDEN) MUTATIONS]

SNP Analysis and Presentation in the Pharmacogenetics of Membrane Transporters Project. DOUG STRYKE et al. PDF - [Read SNP Analysis and Presentation in the Pharmacogenetics of Membrane Transporters Project]

SNP Cutter: SNP PCR-RFLP Assay Design. By Ruifang Zhang, Zanhua Zhu, Fengxia Yao, Hongming Zhu, Tu Nguyen, Chunyu Liu - [Read SNP Cutter: SNP PCR-RFLP Assay Design]

SNP detection with mutagenic primers. Input sequence will be searched to find changes in one nucleotide near the location of the SNP, so that mutagenic primers may be easily designed. Bikandi, J., San Millán, R., Rementeria, A., and Garaizar, J. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR, and endonuclease restriction. Bioinformatics 2004 Mar 22;20(5):798-9. - [Read SNP detection with mutagenic primers]

SNP PCR Primer Design. Chang Biosciences. - [Read SNP PCR Primer Design]

Compare restriction patterns of two sequences for SNP/Mutation detection. Online program that compares restriction enzyme digest of DNA and finds the differences between two sequences. - [Read SNP Restriction Digestion Comparison]

Genetic mapping and manipulation: Chapter 4-SNPs: Introduction and two-point mapping. The SNP database and other information. wormbook.org - [Read SNPs: Introduction and two-point mapping]

TaqMan® SNP Genotyping Assays. Applied Biosystems - [Read TaqMan® SNP Genotyping Assays]