Agarose Gel DNA Extraction Protocols

DNA purification, isolation and extraction protocols from agarose gel slices. Includes methods and procedures for isolating DNA from within agarose gels.

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Agarose Gel DNA Recovery Protocol - http://rothlab.ucdavis.edu/protocols/DNA-from-Agarose-Review.html

Agarose Gel DNA Recovery Protocol - Recovering DNA from agarose gels. Paul N. Hengen, Ph.D - [Read Agarose Gel DNA Recovery Protocol]

DNA Extraction from Agarose Gels Protocol - http://www.methodbook.net/dna/gelextrc.html

DNA Extraction from Agarose Gels Protocol. The page includes cutting out the DNA band from the gel, and describes three methods including 1) Spin-columns (Nucleic acid purification columns), 2) using Dialysis tubing (semi-permeable membrane, Visking tubing), and the 3) Paper strip method.Matt Lewis, Department of Pathology, University of Liverpool. - [Read DNA Extraction from Agarose Gels Protocol]

DNA Fragment Purification from Agarose Protocol - http://axon.med.harvard.edu/~cepko/protocol/mike/D5.html

DNA Fragment Purification from Agarose Protocol. This protocol is best for fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. - [Read DNA Fragment Purification from Agarose Protocol]

DNA Purification from DEAE Nitrocellulose Membranes Protocol - http://rothlab.ucdavis.edu/protocols/P01.html

DNA Purification from DEAE Nitrocellulose Membranes Protocol. Jeff Lawrence - [Read DNA Purification from DEAE Nitrocellulose Membranes Protocol]

Glass Milk Agarose Gel DNA Extracton Protocol - http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/glass_milk.html

Isolation of DNA fragments using glass milk (GENE-CLEAN). Glass Milk Agarose Gel DNA Extracton Protocol. Minion Lab, College of Veterinary Medicine at Iowa State University. - [Read Glass Milk Agarose Gel DNA Extracton Protocol]

Phenol-based Protocol Isolation of DNA Fragments from Low-Melting Temperature Agarose - http://wheat.pw.usda.gov/~lazo/methods/101/101lmtagar.html

Phenol-based Protocol Isolation of DNA Fragments from Low-Melting Temperature Agarose. Reference: Favre, D. 1992. Biotechniques vol. 13. - [Read Phenol-based Protocol Isolation of DNA Fragments from Low-Melting Temperature Agarose]

Rapid Elution of DNA Agarose Gels Protocol - http://www.fhcrc.org/science/labs/hahn/methods/mol_bio_meth/dna_spin_ext.html

Rapid Elution of DNA Agarose Gels Protocol. This method allows quick purification of DNA fragments from agarose gels for use in cloning and other reactions. Higher yields of purified DNA can be obtained from commercially available purification kits, however greater ligation efficiencies per given amount of DNA have been seen with the use of these described spin columns. Hahn Lab. - [Read Rapid Elution of DNA Agarose Gels Protocol]