DNA cloning Procedures Agarose gel purification,
Annealing and extending, oligonucleotides, Ethanol Precipitation, Ligation
Miniprep, Oligonucleotide purification, Recovering DNA bands, Restriction digest
Gene Clean. The Hu Lab.
EMBL Cloning protocols Preparation of oligo solutions
PCR experiments, Digestion of insert DNA, Digestion and dephosphorylation of vector DNA, Ligation of DNA fragments with sticky ends, Ligation of DNA fragments with blunt ends,
Preparation of chemically competent E. coli
The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.