Formaldehyde cross-linking and chromatin immunoprecipitation assays of
tissue culture cells, Based on Boyd and Farnham. Michelle Kallesen, Rosen Lab. - [Read ChIP Assay Protocol PDF]
ChIP on ChIP Protocol PDF- http://genomecenter.ucdavis.edu/farnham/farnham/protocols/MOJO%20Dec%202003%20ChIP-chip%20protocol.pdf
Method to identify genomic targets of DNA-binding
factors. Chromatin
immunoprecipitation (ChIP) assay on high-throughput microarray based methods for
discovering genomic regions occupied by human DNA-binding proteins. Oberley and Farnham. - [Read ChIP on ChIP Protocol PDF]
ChIP ping for Dummies- http://mcardle.oncology.wisc.edu/sugden/Protocols/html%20files/ChIPping%20for%20Dummies.htm
Very nice and detailed ChIP assay protocol procedure. Blocking Staph A cells, Crosslinking cells, Chromatin preparation from cell pellets, sonication, blocking. Sugden Laboratory - [Read ChIP ping for Dummies]
ChIP assay protocol with 2 steps: in vivo formaldehyde cross-linking of whole cells and protein-protein and protein-DNA interactions, followed by immunoprecipitation of protein-DNA complexes with specific antibodies from sonicated extracts. Breeden Lab - [Read Chromatin IP (CHIP assay)]
Mapping Protein/DNA Interactions by Cross-Linking Examining the Distribution of Telomeric and DNA Repair Proteins by ChrIP and Real-Time PCR - [Read Chromatin-IP (ChrIP) Protocol]
Mirmira Lab ChIP Protocol. 50-step protocol forformaldehyde crosslinking to ChIP and also provides recipes for all buffer reagents. - [Read Mirmira Lab ChIP Protocol]