RNA In Situ Hybridization Protocols

Protocols and information related to RNA in situ hybridization.

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mRNA In Situ Hybridization with Nick-Translated Probes Protocol - http://spectorlab.cshl.edu/in_situ_hybridization.html

Protocol for mRNA in situ hybridization with nick-translated probes. - [Read mRNA In Situ Hybridization with Nick-Translated Probes Protocol]

RNA in situ Probe Synthesis Protocol - http://www.fhcrc.org/science/labs/moens/Protocols/probe.html

Protocol for RNA in situ probe synthesis. Includes:DNA Template Preparation; Probe Synthesis; Probe Purification using a Sephadex G-50 Spin Column; Probe Hydrolysis. - [Read RNA in situ Probe Synthesis Protocol]

RNA Labeling by In Vitro Transcription of DNA with DIG, Biotin or Fluorescein RNA Labeling Mix - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_54-57.pdf

Protocol for RNA labeling by in vitro transcription of DNA with DIG, Biotin or Fluorescein RNA Labeling Mix. A PCR fragment that has the appropriate promoter ligated to its 5’-ends can also serve as a transcription template. The procedure described incorporates one modified nucleotide (DIG-, Biotin-, or Fluorescein-UTP) at approximately every 20 – 25th position in the transcripts. - [Read RNA Labeling by In Vitro Transcription of DNA with DIG, Biotin or Fluorescein RNA Labeling Mix]

RNA-RNA In Situ Hybridization Using DIG-Labeled Probes Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_172-177.pdf

RNA-RNA in situ hybridization using DIG-labeled probes: the effect of high molecular weight polyvinyl alcohol on the alkaline phosphatase indoxyl-nitroblue tetrazolium reaction. RNA-RNA in situ hybridization protocol using alkaline phosphatase-conjugated digoxigenin-(DIG-) labeled probes is presented. The addition of polyvinyl alcohol (PVA) of high molecular weight (40 – 100 kD) to the BCIP-NBT detection system enhances the alkaline phosphatase reaction and prevents... - [Read RNA-RNA In Situ Hybridization Using DIG-Labeled Probes Protocol]

Visualizing miRNAs in Adult Skeletal Muscle by Hybridization in situ Protocol - http://www.natureprotocols.com/2006/06/29/visualizing_mirnas_in_adult_sk.php

Protocol for visualizing miRNAs in adult skeletal muscle by hybridization in situ. Includes: Visualizing miRNAs in adult skeletal muscle by hybridization in situ; Probe preparation; Tissue preparation; Fixation; Deproteinization with Proteinase K; Acetylation of the tissue; Dehydration; Hybridization step; Post-Hybridization Washes (Step 1-6 should be performed on shaker); Signal Detection (Steps should be performed on a shaker); Post-Detection Washes. - [Read Visualizing miRNAs in Adult Skeletal Muscle by Hybridization in situ Protocol]