Slide Preparation for Cytogenetics Protocol Protocol for slide preparation for cytogenetics. Includes: Improved cell recovery by microcentrifugation; Slide storage; "Controlled" chromosome spreading; Dropping cells from a height does not improve spreading; GTG-banding.
Staining Chromosomes (G-Banding) Protocol Protocol for staining chromosomes (G-Banding). Includes: Trypsin (1:250) USP Grade; Gurr Buffer Solution: (1 liter); Working Salt Solution: (1 liter); Leishman Stain Solution.
Studying Mitosis in Cultured Mammalian Cells Protocol Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. Another inexpensive and easy-to-use alternative is to grow cells in a culture dish with a glass bottom. Such dishes are suitable for microinjection experiments.
