Fluorescent In Situ Hybridization FISH Protocol

Additional slide pre-treatment for mapping smaller insert clones for FISH. Sanger Institute. - [Read Additional slide pre-treatment for mapping smaller insert clones]

Alexa Dyes, Cy Dyes and other Fluors: succinimidyl esters. - [Read Alexa Dyes, Cy Dyes and Other Fluors: Succinimidyl Esters]

Bacterial clone DNA Protocol for FISH. - [Read Bacterial clone DNA Protocol for FISH]

Protocol for BioPrime biotin labeling (FISH probe). - [Read BioPrime Biotin Labeling (FISH probe) Protocol]

Protocol for chromosome preparation and Fluorescence in-situ hybridization (FISH). Chromosome preparation and Fluorescence in-situ hybridization (FISH). - [Read Chromosome Preparation and Fluorescence In-Situ Hybridization (FISH) Protocol]

Protocol for cryptic translocation identification in human and mouse using several telomeric multiplex FISH (TM-FISH) strategies. - [Read Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH]

Protocol for custom fluorescent nucleotide synthesis/nucleic acid labeling. - [Read Custom Fluorescent Nucleotide Synthesis/Nucleic Acid Labeling Protocol]

Protocol for Detection (FISH). TRITC, or Avidin Cy-5. For the probes labeled with digoxigenin, we usually first incubate with mouse-anti-digoxigenin, followed by incubation with sheep anti-mouse Cy5.5, or other fluorochrome conjugated antibodies. - [Read Detection FISH Protocol]

DNA FIBERS FISH Protocol and Methods. Sanger Institute. - [Read DNA FIBERS FISH]

Protocol for DNA precipitation and hybridization (FISH). Includes: Precipitation; Slide Denaturation and Hybridization. - [Read DNA Precipitation and Hybridization FISH Protocol]

FISH Guide and Troubleshooting. Includes: Cytogenetic techniques; Slide preparation; When do chromosomes spread; Cell suspensions; G-banding; Cell fixatives; Slide preparation factors. - [Read FISH Guide and Troubleshooting]

FISH Hybridization Protocol. Sanger Institute. - [Read FISH Hybridization Protocol]

Protocol for fluorescence in situ hybridization (FISH) for DNA replication origins. Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique used for the detection of specific chromosomal rearrangements and applicable to many different specimen types. FISH is widely used for several diagnostic applications. - [Read Fluorescence In Situ Hybridization (FISH) for DNA Replication Origins Protocol]

Protocol for fluorescence in situ hybridization (FISH) on human chromosomes and interphase nuclei. Includes: Plasmid probes; COSMID-, YAC- and LIBRARY PROBES; Probe detection. - [Read Fluorescence In Situ Hybridization (FISH) on Human Chromosomes and Interphase Nuclei Protocol]

Protocol for fluorescence in situ hybridization of a repetitive DNA probe to human chromosomes in suspension. Hybridization technique which does not need formamide and dextran sulfate. As a model system, we used the repetitive specific human DNA probe pUC 1.77, labeled it with digoxigenin-11-dUTP by nick-translation, and hybridized it to metaphase chromosomes in suspension. These chromosomes were isolated by standard techniques from human lymphocytes. - [Read Fluorescence In Situ Hybridization of a Repetitive DNA Probe to Human Chromosomes in Suspension]

Method assesses cellular mRNA transcripts in tissue sections and cell cultures using unique short anti-sense primers directed against sequences in particular protein(s). The unlabeled synthetic cDNA oligonucleotide primers are extended complementary to a sense mRNA transcript using reverse transcriptase and labeled through incorporation of a fluorescent-labeled dUTP nucleotide base. This procedure provides rapid detection of low abundance mRNA messages that can be related to other cellular.... - [Read Fluorescent In Situ Transcription in Cells and Tissues Protocol]

Hybridisation of Interphase Nuclei Method. Sanger Institute. - [Read Hybridisation of Interphase Nuclei Protocol]

Interphase nuclei preparation for FISH. Sanger Institute. - [Read Interphase nuclei]

Protocol for Nick Translation FISH. - [Read Nick Translation FISH Protocol]

Nick translation for FISH. Sanger Institute. - [Read Nick translation for FISH]

Preparation of metaphase chromosomes from lymphoblastoid cell lines for FISH analysis. Sanger Institue. - [Read Preparation of metaphase chromosomes from lymphoblastoid cell lines]

Protocol for the preparation of paraffin sections and frozen tissue for FISH. - [Read Preparation of Paraffin Sections and Frozen Tissue for FISH Protocol]

Protocol for the preparation of stock solutions for FISH. - [Read Preparation of Stock Solutions for FISH Protocol]

Protocol for the pretreatment of chromosome slides for FISH/CGH/SKY. - [Read Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol]

Single colour detection for biotinylated probes. Sanger Institute - [Read Single Colour Fish Analysis Method]