S Phase Protocols

The S phase or synthesis phase, is a period in the cell cycle during interphase, between G1 phase and the G2 phase. Following G1, the cell enters the S stage, when DNA synthesis or replication occurs. Find protocols here related to S phase.

Protocols » Cell Biology and Cell Culture » Cell Cycle Protocols » S Phase Protocols

S Phase Protocols Protocol Links Sort by: Hits | Alphabetical

G1/S Phase Synchronization using Double Thymidine Synchronization Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4487

This protocol provides a method for synchronizing cells at the G1/S border using a double treatment of thymidine, which, in excess, is an inhibitor of DNA synthesis. Cells are treated once with excess thymidine to accumulate the majority of them at G1/S; however, some cells will have stopped growth within the S phase. - [Read G1/S Phase Synchronization using Double Thymidine Synchronization Protocol]

G1/S Phase Synchronization Using Mimosine Arrest Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4488

Protocol uses the plant amino acid mimosine as a G1/S synchronizing agent. Cells are first treated with excess thymidine to accumulate the majority of them at G1/S; however, some cells will have stopped growth within the S phase. Thymidine is then removed to allow all the cells to proceed completely through the S phase. Mimosine is then added to arrest the cells at the G1/S border. When mimosine is removed, cells will begin to enter S phase within about 1 hour. - [Read G1/S Phase Synchronization Using Mimosine Arrest Protocol]