The G phase is a period in the cell cycle during interphase, after cytokinesis and before the S phase. This phase is the major period of cell growth during the cell’s lifespan. It is during this stage new organelles are being synthesized the cell therefore requires both structural proteins and enzymes, resulting in great amount of protein synthesis. Find protocols here related to the G phase.
G1 Synchronization of CHO Cells by Isoleucine Deprivation Protocol This protocol provides a method for the synchronization of a monolayer culture of CHO cells in G1 using isoleucine deprivation. Since CHO cells can also be adapted to grow in suspension culture, this procedure can be used to obtain larger quantities of cells. When isoleucine is replaced, the cells resume growth and begin to enter S phase ~4 hours later. This method arrests almost 100% of the CHO cells in G1, and upon reversal, leads to rapid recovery of cell growth and very high cell viability.
G1/S Phase Synchronization using Double Thymidine Synchronization Protocols This protocol provides a method for synchronizing cells at the G1/S border using a double treatment of thymidine, which, in excess, is an inhibitor of DNA synthesis. Cells are treated once with excess thymidine to accumulate the majority of them at G1/S; however, some cells will have stopped growth within the S phase.
G1/S Phase Synchronization Using Mimosine Arrest Protocol This protocol uses the plant amino acid mimosine as a G1/S synchronizing agent. Cells are first treated with excess thymidine to accumulate the majority of them at G1/S; however, some cells will have stopped growth within the S phase. Thymidine is then removed to allow all the cells to proceed completely through the S phase. Mimosine is then added to arrest the cells at the G1/S border. When mimosine is removed, cells will begin to enter S phase within about 1 hour.