Welcome to Molecular Station!

You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

Already registered? Login now below.

User Name:

Password:

Remember Me?

Already registered and Forgot your password? Click below to recover it.

Recover Lost Password

Join now - it's fast and free!

Molecular Station is THE largest network of researchers, scientists and science lovers anywhere!

Recent Forum Posts

Western Blot Optimization

This is your portal for optimizing western blots!

Western Blot Optimization Table of Contents

• What is Western Blot Optimization?
• Factors in Membrane Optimization
• Applications
• Discussion Topics for Western Blot Optimization
• Get Help on your Western Blot Optimization Problem

What is Optimization of Western Blots?

Optimization of western blots is important as this is done to increase the signal to noise ratio of your results, and also decrease non-specific bands. Optimization is carried out usually in a trial and error or a more organized approach by using different blocking, incubation and washing conditions.

Factors in Western Blot Optimization

Several factors are key in optimizing Western Blots:

• SDS-PAGE Gel electrophoresis - without a good gel you will not get a great membrane
• Selecting the Right Antibodies (both primary and secondary) and Using them Properly (dilution is important)
• Blocking Conditions
• Washing Conditions
• Development Condition

Factors in Western Blot Optimization

Several factors are key in optimizing Western Blots:

• SDS-PAGE Gel electrophoresis - without a good gel you will not get a great membrane
• Selecting the Right Antibodies (both primary and secondary) and Using them Properly (dilution is important)
• Blocking Conditions
• Washing Conditions
• Development Condition