Membrane Stripping and Re-probing Protocol - Western Blotting
Membrane Stripping and Reprobing Protocol for Western Blotting
Stipping and re-probing blots
- add 280 μl of 2-beta-mercaptoethanol per 40 mL of stripping buffer (to a final concentration of 100 mM)
- Wash membrane in TBST (1 X 5 min)
- Incubate membrane in stripping buffer for 30 min at 50-60°C (use a heating oven for this - and keep the oven door closed due to smell)
- Wash in TBST (2 X 5 min)
- Block membrane for 1 h in 5% blocking milk
- Follow normal protocol for developing western blots.
- Membrane Stripping Buffer
Recipe:
- Stripping buffer: 62.5 mM Tris-HCl (pH 6.8), 2% SDS. (add 280 ul of 2-beta merceptoethanol or a final concentration of 0.1 M 2-Mecaptoethanol).
- See our Western Blotting Protocol
Other Protein Protocols in our Protocol Database
Our Protein Protocols
![[أربيك]](http://www.molecularstation.com/translate/images/flag_ar.gif){kind=small}
Send this page to a friend
