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PCR Troubleshooting

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PCR Troubleshooting

Polymerase Chain Reaction Problems and Solutions

This is a troubleshooting page for common PCR problems and their solutions.

PCR Problem

Long Non-Specific PCR Products

PCR Primer Dimers

 

 

Factors for a Successful PCR

Can't Find Your Problem? Make sure you see the PCR Forum for PCR troubleshooting and solutions to common problems. You can also post questions to other researchers by simply registering and then clicking on Post New Thread or the question mark below!

PCR Forum Topics

New to PCR techniques Hello there! my name is 'Dare and I'm doing my undergraduate project on Genetic characterization of chickens with mtDNA. I would really appreciate...
PCR Troubleshooting Guide Hi everyone, we are trying to setup a PCR Troubleshooting Guide. Please help by posting common problems and their solutions here. thanks:notworthy:
PCR contamination I have a problem during PCR program. add templete DNA, dNTP, primer, polymerase, etc..to ep tube. then I forgot to add oiling U-U:mellow: so.. I...
PCR, incomplete products? Hello, I am using iproof high-fedility polymerase to amplify a DNA fragment 6kb in size from a genome. With Taq that's almost impossible but iproof...
PCR Inhibition by DNA HELLO ALL! I have a big pcr problem. I am trying to conduct a pcr using plasmid DNA I purified using a Qiagen midi kit. I am using this DNA to make...
plz help in these questions 1-What dNTP do for PCR ? 2- My PCR samples stay at the top of the agarose gel while the ladder showed a complete run?
Re-PCR trouble Hello everyone. I am trying to amplify a gene, and at the same time to introduce point mutations, to enable the digestion with a specific enzyme of...
Volume Loss during PCR?!! So I run my PCR tubes for 30 cycles with a volume of 15ul. All capped tightly, but I end up with 8-10 ul volume after PCR. I tried spinning them down...
Rt-pcr rtpcr -------------------------------------------------------------------------------- while doing rt pcr when i am using 20pM conc. of primer i...
pcr smear Hi, i want to know what are the causes of pcr smear. coz i have been having some smear from last one week , but early it workd great... i even tried...
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