PCR Troubleshooting

Polymerase Chain Reaction Problems and Solutions

This is a troubleshooting page for common PCR problems and their solutions.

PCR Problems & Troubleshooting Guides

Long Non-Specific PCR Products

PCR Primer Dimers

PCR No Bands

PCR Non Specific Product

Questions & Troubleshooting Forum Discussions

Yeast Colony PCR Troubleshooting
RT-PCR Troubleshooting - HELP!
RT-PCR Troubleshooting - HELP!
PCR troubleshooting, Pfu Ultra II, whole worm DNA
PCR Troubleshooting Guide
PCR troubleshooting for molecular cloning
PCR troubleshooting - Product streaks and primer dimer formation
PCR troubleshooting - no product
PCR troubleshooting

Factors for a Successful PCR

Can't Find Your Problem? Make sure you see the PCR Forum for PCR troubleshooting and solutions to common problems. You can also post questions to other researchers by simply registering and then clicking on Post New Thread or the question mark below!

PCR Forum Topics

Heated lid on RFLP: is it ok? I'm doing three different RFLPs with three different restriction enzymes using a single amplicon, each RFLP in different tubes of course as each...
Real time PCR, issue normalization Hello all, I am new to the forum and posting because I can't seem to find anyone in my lab which does work on qPCR. I am interested in getting...
Velocity DNA Polymerase Vs MyTaq Could anyone help me with finding out the difference between using MyTaq DNA Polymerase and Velocity and is there any advantages/disadvantages each...
Real Time PCR DNA Concentration I have a set of mtDNA Taqman assays for various animal species and I'm trying to validate them. Because this assay is qualitative, I have not been...
Sequencing whole cDNA using PCR Since I'm a bit of a novice at molecular biology, I thought I would ask before spending a lot of money on kits and reagents in order to try to...
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