PCR Troubleshooting

Polymerase Chain Reaction Problems and Solutions

This is a troubleshooting page for common PCR problems and their solutions.

PCR Problem

Long Non-Specific PCR Products

PCR Primer Dimers

Factors for a Successful PCR

Can't Find Your Problem? Make sure you see the PCR Forum for PCR troubleshooting and solutions to common problems. You can also post questions to other researchers by simply registering and then clicking on Post New Thread or the question mark below!

PCR Forum Topics

3M Integrated Cycler Validation I just had a demo for the 3M integrated cycler yesterday. It looks like we're going to pick it up for infectious disease diagnosis and use a...
qPCR please help Heya, I am having problems with my qPCR. I am trying to amplify the humand ADH1A gene. I have primers (do not know the sequence) that seem to work...
real time data analysis problem hey guys i wonder if anyone can help me. im trying to determine siRNA knockdown using RTPCr, does anyone know how to interpret data whereby the...
Need Manual for Perkin Elmer GeneAmp 9600 Hey guys, so one of my coworkers misplaced the manual for our Perkin Elmer GeneAmp 9600 and I was hoping to download a replace from the internet....
Why can the polymerase chain reaction only be used to amplify genes that have... ...been cloned and sequenced? The polymerase chain reaction can only be used to amplify genes that have already been cloned and sequenced. Why is...
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