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New to PCR techniques Hello there! my name is 'Dare and I'm doing my undergraduate project on Genetic characterization of chickens with mtDNA. I would really appreciate...
PCR Troubleshooting Guide Hi everyone, we are trying to setup a PCR Troubleshooting Guide. Please help by posting common problems and their solutions here. thanks:notworthy:
PCR contamination I have a problem during PCR program. add templete DNA, dNTP, primer, polymerase, etc..to ep tube. then I forgot to add oiling U-U:mellow: so.. I...
PCR, incomplete products? Hello, I am using iproof high-fedility polymerase to amplify a DNA fragment 6kb in size from a genome. With Taq that's almost impossible but iproof...
PCR Inhibition by DNA HELLO ALL! I have a big pcr problem. I am trying to conduct a pcr using plasmid DNA I purified using a Qiagen midi kit. I am using this DNA to make...
plz help in these questions 1-What dNTP do for PCR ? 2- My PCR samples stay at the top of the agarose gel while the ladder showed a complete run?
Re-PCR trouble Hello everyone. I am trying to amplify a gene, and at the same time to introduce point mutations, to enable the digestion with a specific enzyme of...
Volume Loss during PCR?!! So I run my PCR tubes for 30 cycles with a volume of 15ul. All capped tightly, but I end up with 8-10 ul volume after PCR. I tried spinning them down...
Rt-pcr rtpcr -------------------------------------------------------------------------------- while doing rt pcr when i am using 20pM conc. of primer i...
pcr smear Hi, i want to know what are the causes of pcr smear. coz i have been having some smear from last one week , but early it workd great... i even tried...
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