PCR Troubleshooting

Polymerase Chain Reaction Problems and Solutions

This is a troubleshooting page for common PCR problems and their solutions.

PCR Problem

Long Non-Specific PCR Products

PCR Primer Dimers

Factors for a Successful PCR

Can't Find Your Problem? Make sure you see the PCR Forum for PCR troubleshooting and solutions to common problems. You can also post questions to other researchers by simply registering and then clicking on Post New Thread or the question mark below!

PCR Forum Topics

PCR Reaction + Inhibitors Hello, I'm having some difficulty with PCR inhibition. I'm past the point of optimizing my PCR reaction, and I am currently screening my organisms...
Degenerate primers Thanks Aga, Actually the degenerate primers were designed by multiple sequence alignment of protein sequences of target gene in different bacteria....
10x PCR buffer and beta-mercaptoethanol Hi, I just want to know if anyone adds beta-mercaptoethanol to their 10x PCR buffer and if so how much 10 mM beta-mercaptoethanol would need to be...
qRT-PCR and TaqMan gadd45 Hello! Did anyone work with gadd45 and its mRNA expression? I wonder about the high or low abundance of gadd45 in normal cells? I know that 28s or...
How can RT-PCR(reverse transcriptase polymerase chain reaction)be used to ass... splicing of specific mRNA? I know the very basics of how RT-PCR works so I am not really sure how this would relate to alternatvie splicing. Any...
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