Bioinformatics, Protocols, DNA RNA Protein Proteomics
 |
|---|
You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!
Already registered? Login now below.
Already registered and Forgot your password? Click below to recover it.
Join now - it's fast and free!
Molecular Station is THE largest network of researchers, scientists and science lovers anywhere!
A fact is a simple statement that everyone believes. It is innocent, unless found guilty. A hypothesis is a novel suggestion that no one wants to believe. It is guilty, until found effective. ~Edward Teller
In our opinion the most important methodological invention in molecular biology has been the polymerase chain reaction (PCR) as it has allowed a literal explosion of molecular biology and genetics advancements through the cloning of genes.
please help me-basic information about site-directed mutagenesis PCR
I have to change a codon in my plasmid vector. It should be changed by site-directed mutagenesis.
Now I know that i made any mistakes. I have no...
Nanodrop
Hello all
Is there anyone out there who uses Genecelan III kit for PCR product purification? If so, could you quantify that using Nanodrop?
Thanks
Rt Pcr
After doing PCR i got very light band of IL-3.Plz tell me what should i do.should i increase the concentarion of cDNA?or any other suggestion?
primers at room temp.
thx aftab!
Advantages of Thermostable Polymerases in PCR Reactions?
What iare the advantages of using thermostable enzymes in a polymerase chain reaction??
PCR on "dirty" samples
Hi all!
I'm getting very tiny biopsy samples for my PCR analysis. I tried to purify DNA from these samples using conventional methods, adding...
You must REGISTER NOW to post a question in the PCR Forum by clicking 
here.
View More PCR discussions and troubleshooting in the: PCR Forum
We really recommend you read our excellent review of the History of the Polymerase Chain Reaction (PCR)
PCR is a primer-mediated enzymatic amplification of specifically cloned or genomic DNA sequences. It has become a routine procedure in every molecular biology lab for manipulating and identifying genetic material. New applications have now been generated with remarkable regularity. These applications are transforming molecular biology.
In the post genomic era, PCR had become the method of choice to clone existing genes and generate a wide array of new genes by mutagenesis and recombination within the genes of interest. The quick and easy availability of these genes is crucial for the study of functional genomics, protein structure function relationships, protein engineering, protein protein interactions, gene expression and essentially molecular evolution.
 |
 |
 |
 |
 |
|
|---|---|---|---|---|---|
PCR Protocols
|
PCR Bioinformatics and Databases |
Learn about PCR |
PCR Kits and Products |
PCR Forum |
PCR Books
|
For a successful PCR you need the following (read the following articles to improve and learn PCR):
Template DNA or sample starting material
Please read our review of the History of PCR
PCR Primer Design Guidelines and Software
Review on the History and Development of the Polymerase Chain Reaction (PCR)
Also visit PCR Station
Polymerase Chain Reaction - PCR
Other Types of PCR - Related:
Disclaimer / Terms of Service |
Privacy Policy|
©2005-2007 Molecular Station.com, All rights reserved.
Send this page to a friend
