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PCR

Polymerase Chain Reaction

In our opinion the most important methodological invention in molecular biology has been the polymerase chain reaction (PCR) as it has allowed a literal explosion of molecular biology and genetics advancements through the cloning of genes.

PCR Forum Topics

please help me-basic information about site-directed mutagenesis PCR I have to change a codon in my plasmid vector. It should be changed by site-directed mutagenesis. Now I know that i made any mistakes. I have no...
Nanodrop Hello all Is there anyone out there who uses Genecelan III kit for PCR product purification? If so, could you quantify that using Nanodrop? Thanks
Rt Pcr After doing PCR i got very light band of IL-3.Plz tell me what should i do.should i increase the concentarion of cDNA?or any other suggestion?
primers at room temp. thx aftab!
Advantages of Thermostable Polymerases in PCR Reactions? What iare the advantages of using thermostable enzymes in a polymerase chain reaction??
PCR on "dirty" samples Hi all! I'm getting very tiny biopsy samples for my PCR analysis. I tried to purify DNA from these samples using conventional methods, adding...
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We really recommend you read our excellent review of the History of the Polymerase Chain Reaction (PCR)

PCR is a primer-mediated enzymatic amplification of specifically cloned or genomic DNA sequences.  It has become a routine procedure in every molecular biology lab for manipulating and identifying genetic material.  New applications have now been generated with remarkable regularity.  These applications are transforming molecular biology.
In the post genomic era, PCR had become the method of choice to clone existing genes and generate a wide array of new genes by mutagenesis and recombination within the genes of interest.  The quick and easy availability of these genes is crucial for the study of functional genomics, protein structure function relationships, protein engineering, protein protein interactions, gene expression and essentially molecular evolution.

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PCR Guidelines:

For a successful PCR you need the following (read the following articles to improve and learn PCR):

Template DNA or sample starting material

PCR primers

PCR Enzymes

Factors for a Successful PCR

Post-PCR Analysis:

Troubleshooting PCRs

Analysis of PCR Reactions

 

Please read our review of the History of PCR

Articles and Information on PCR

PCR Polymerases

Avoiding PCR Contamination

PCR Primer Design Guidelines and Software

Review on the History and Development of the Polymerase Chain Reaction (PCR)

Also visit PCR Station

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Polymerase Chain Reaction - PCR

 

Other Types of PCR - Related:

Real-Time PCR

 

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