PCR

Polymerase Chain Reaction

In our opinion the most important methodological invention in molecular biology has been the polymerase chain reaction (PCR) as it has allowed a literal explosion of molecular biology and genetics advancements through the cloning of genes.

PCR Articles

PCR Troubleshooting

Factors for Successful PCR

PCR Based Site Directed Mutagenesis

Analysis of PCR Reactions

Real Time PCR

PCR Protocols

Colony PCR Protocols

PCR Optimization Protocols

PCR Troubleshotting Protocols

Standard PCR Protocol

In Situ PCR Protocols

AFLP PCR Protocols

Real Time PCR Protocols

PCR Forum Topics

Detecting changes in GATA3 expression RT-PCR taq Hi all, Here is a puzzler for you. I am attempting to create a positive control for when i am measuring the expression of GATA3 in activated T...
4student for test bank and solution manual services:4student.com@gmail.com 4student for test bank and solution manual services:4student.com@gmail.com thanks
genome walker kit Hi, Can anyone suggest a good genome walker kit? I know a piece of sequence and interested in walking at-least 2 kb upstream and downstream of my...
Unpredictable, changing PCR results Hi Everybody. I'm having major problems with my pcr (like many others... :S), and now i'm running out of ideas, so i hope some of you could help me...
pcr hello, i had pcr and i saw an unespeciefic band in electrofores gels, i changed pcr buffer, dna, dntp and primers and i found the unespecified band...
You must REGISTER NOW to post a question in the PCR Forum by clicking here.

View More PCR discussions and troubleshooting in the: PCR Forum

PCR Encyclopedia

PCR Pictures

Welcome Back, Unregistered !

Upload Photos

PCR Newsletter

We really recommend you read our excellent review of the History of the Polymerase Chain Reaction (PCR)

PCR is a primer-mediated enzymatic amplification of specifically cloned or genomic DNA sequences. It has become a routine procedure in every molecular biology lab for manipulating and identifying genetic material. New applications have now been generated with remarkable regularity. These applications are transforming molecular biology.
In the post genomic era, PCR had become the method of choice to clone existing genes and generate a wide array of new genes by mutagenesis and recombination within the genes of interest. The quick and easy availability of these genes is crucial for the study of functional genomics, protein structure function relationships, protein engineering, protein protein interactions, gene expression and essentially molecular evolution.

Diagram 1 - PCR Cycle Steps

PCR

Steps in a PCR Reaction

There are 3 steps in one PCR cycle reaction (see diagram 1 - PCR Cycle Steps). In step 1, the DNA is denatured at high temperatures (from 90 - 97 degrees celcius). In step 2, primers anneal to the DNA template strands to prime extension. In step 3, extension occurs at the end of the annealed primers to create a complementary copy strand of DNA. This effectively doubles the DNA quantity through the 3 steps in the PCR cycle. This cycle can repeat indefinitely theoretically, however normally constitutes 25-40 cycles.