Welcome to Molecular Station!

You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

Already registered? Login now below.

Already registered and Forgot your password? Click below to recover it.

Recover Lost Password

Join now - it's fast and free!

Molecular Station is THE largest network of researchers, scientists and science lovers anywhere!

Molecular Biology - Science Quotes

There is no prescribed route to follow to arrive at a new idea. You have to make the intuitive leap. But the difference is that once you've made the intuitive leap you have to justify it by filling in the intermediate steps. In my case, it often happens that I have an idea, but then I try to fill in the intermediate steps and find that they don't work, so I have to give it up. ~Stephen W. Hawking

Molecular Biology Newsletter!

Recent Forum Posts

Gene Cloning

Information on how genes, (usually proteins) or cells are cloned.

Gene Cloning Method

The purpose of any cloning experiment is to produce a clone, a group of identical cells or organisms. We know that some plants can be cloned simply by taking cuttings, and that others can be cloned by growing whole plants from single cells collected from one plant.

Even vertebrates can be cloned. John Gurdon produced clones of identical frogs by transplanting nuclei from a single frog embryo to many enucleate eggs, and a sheep named Dolly was cloned in Scotland in 1997 using an enucleate egg and a nucleus from an adult sheep mammary gland. Identical twins constitute a natural clone.

The procedure we usually follow in a gene cloning experiment is to place a foreign gene into a bacterial cell, then to grow a clone of these modified bacteria, with each bacterial cell containing the foreign gene. Thus, as long as we ensure that the foreign gene can replicate, we can clone the gene by cloning its bacterial host. Stanley Cohen, Herbert Boyer, and their colleagues performed the first cloning experiment in 1973.

Bioinformatics, Protocols, DNA RNA Protein Proteomics

Intracellular Stations