Western Blot Western izbrisat
Membrane Stripping and Re-probing Protocol - Western Blotting Membranski raskrivanje i Re-probinga Protokol - Western bloting
Membrane Stripping and Reprobing Protocol for Western Blotting Membranski raskrivanje i Reprobing protokola za Western bloting
Stipping and re-probing blots Stipping i re-probinga blots
- add 280 μl of 2-beta-mercaptoethanol per 40 mL of stripping buffer (to a final concentration of 100 mM) dodati 280 μ l, 2-beta-mercaptoethanol po 40 mL i raskrivanje međuspremnik (do konačne koncentracije od 100 mm)
- Wash membrane in TBST (1 X 5 min) Praona membrana u TBST (1 x 5 min)
- Incubate membrane in stripping buffer for 30 min at 50-60°C (use a heating oven for this - and keep the oven door closed due to smell) Izleći piliće membrana u raskrivanje buffer za 30 min na 50-60 ° C (koristimo grijanje peći za ovu - i zadržite pećnica vrata zatvorena zbog mirisa)
- Wash in TBST (2 X 5 min) Opere u TBST (2 x 5 min)
- Block membrane for 1 h in 5% blocking milk Blokiranje membrane za 1 h za 5% za blokiranje mlijeko
- Follow normal protocol for developing western blots. Slijedite uobičajeni protokol za razvoj zapadne blots.
- Membrane Stripping Buffer Recipe: Membranski raskrivanje Buffer Recept:
- Stripping buffer: 62.5 mM Tris-HCl (pH 6.8), 2% SDS. Raskrivanje tampon: 62,5 mm-Tris HCL (pH 6,8), 2% SDS-a. (add 280 ul of 2-beta merceptoethanol or a final concentration of 0.1 M 2-Mecaptoethanol). (dodati 280 ul. 2-beta merceptoethanol ili konačni koncentraciji 0,1 M 2-Mecaptoethanol).
- See our Western Blotting Protocol See our Western bloting Protocol
Other Protein Protocols in our Protocol Database Ostalo protein Protokoli u našem Protokol baze podataka
Our Protein Protocols Naš protein Protocols
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