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PCR Protocols PCR Protocols	PCR Bioinformatics and Databases PCR bioinformatiku i baze podataka	Learn about PCR Saznajte više o PCR	PCR Kits and Products PCR Kits i proizvodi	PCR Forum PCR Forum	PCR Books PCR Knjige

PCR Primer Design PCR Primer Design

Primers for PCR Početnice za PCR

Guidelines for primer selection: Smjernice za upaljač za odabir:

• PCR primers are designed as 15-30 oligo nucleotides in length. PCR početnice su razrađene kao 15-30 oligo nukleotidima u dužini. Longer primers usually allow higher specificity or allow the addition of restriction enzyme sites to the primer end for cloning. Više početnice obično dopuštaju veći specifičnost ili dozvoliti priključivanje ograničenje enzim sučelja na kraju upaljač za kloniranje.
• Primer oligonucleotide GC content should be in the range of 40-60%. Udžbenik oligonucleotide GC sadržaj treba biti u rasponu od 40-60%. G and C nucleotides should be uniformly distributed as clusters of G's or C's cause non-specific priming may occur and high GC content impairs the PCR efficiency. G i C nukleotidima bi trebao biti jednako distribuiranih kao grozdovi, G ili C je uzrok ne-specifične priming svibanj pojaviti i visoka GC sadržaj narušava se PCR efikasnost.
• Primers should not be self-complementary or complementary to the other primer in the reaction mixture. Početnice ne bi trebao biti self-komplementarni komplementarnih ili na drugi udžbenik u reaction mixture. Primers that complement with each other compete with the template for the use of primer and reagent. Početnice da komplement međusobno natječu s predloška za upotrebu boja i reagens. These are called primer dimers and hairpin formation when a primer self-complements. To se naziva prvom dimeri i hairpin formation kada je fitilj self-nadopunjuje.
• The melting temperature Tm of the primers should not have a difference more than 5°C. The melting temperature Trn u početnice ne bi trebali imati razlika više od 5 ° C.

PCR Primer Design Software Online PCR Primer Design Software Online

Recommended Primer Design Software: Preporučeni Primer Design Software:

WebPrimer Picks the best pair of primers from many conditions which can be set manually. WebPrimer Picks najbolji par početnice iz mnogih uvjete koji se mogu podesiti ručno.

Primer3 Primer3 primer design software picks primers from a DNA sequence. Primer3 Primer3 upaljač dizajn softver pijucima početnice iz DNA sekvence. Picks primers from many conditions which can be set manually. Picks početnice iz mnogih uvjete koji se mogu podesiti ručno.