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Put off your imagination, as you put off your overcoat, when you enter the laboratory. Put od vaše mašte, kao što je odložio vaš ogrtač, kad ulaze u laboratoriju. But put it on again, as you put on your overcoat, when you leave. No, stavi ga na opet, kao što ste stavili na vaš ogrtač, kad vas ostaviti. ~Attributed to Claude Bernard (French physiologist, 1813-1878) ~ Pridodane Claude Bernard (francuski fiziolog, 1813-1878)
The purpose of any cloning experiment is to produce a clone, a group of identical cells or organisms. We know that some plants can be cloned simply by taking cuttings, and that others can be cloned by growing whole plants from single cells collected from one plant. Cilj eksperimenta bilo koji klon je proizvesti klonom, grupa identičan stanica ili organizama. Mi znamo da neke biljke mogu biti klon jednostavno uzimanje reznice, i da drugi mogu biti klon raste po cijele biljke iz jednog stanice prikupljene iz jednog biljka .
Even vertebrates can be cloned. John Gurdon produced clones of identical frogs by transplanting nuclei from a single frog embryo to many enucleate eggs, and a sheep named Dolly was cloned in Scotland in 1997 using an enucleate egg and a nucleus from an adult sheep mammary gland. Identical twins constitute a natural clone. Čak i kralješnjaka može biti klon. John Gurdon proizvedene klonovi od identičan žabe by transplanting jezgre iz jednog žaba embrij mnogim enucleate jaja, i ovce zove Dolly je klon u Škotskoj u 1997 koristeći enucleate jaje i jezgra iz jedne odrasle ovce mammary gland . Identični blizanci čine klon prirodna.
The procedure we usually follow in a gene cloning experiment is to place a foreign gene into a bacterial cell, then to grow a clone of these modified bacteria, with each bacterial cell containing the foreign gene. Thus, as long as we ensure that the foreign gene can replicate, we can clone the gene by cloning its bacterial host. Stanley Cohen, Herbert Boyer, and their colleagues performed the first cloning experiment in 1973. Postupak se obično slijedi u kloniranje gena eksperiment je mjesto stranog gena u bakterijske ćelije, a zatim raste jedan klon od tih izmijenjenih bakterija, bakterija sa svakim stanica sadrži stranih gena. Dakle, dokle god smo osigurali da strani gena može odgovor, u mogućnosti smo klon, klon gena po svojim domaćinom bakterija. Stanley Cohen, Herbert Boyer, i njihove kolege obavili prvi klon eksperiment u 1973.
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