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Electropray Mass Spectrometry Electropray mass spectrometry

What is a Electrospray MS? Što je Electrospray MS?

The Mass of Proteins can be Precisely Determined Electrospray mass spectrometry Masa proteina može biti Upravo određuje Electrospray mass spectrometry

The very low volatility of proteins was a barrier for many years to using mass spectrometry, an estazblished analytical technique in organic chemistry.  This difficulty has been circumvented by the recent introduction of electrospray ionization mass spectrometry.  A protein sample in an acidic volatile solvent is sprayed into a mass spectrometer.  The solvent surrounding individual droplets evaporates rapidly in the vacuum chamber of the instrument, leaving unfragmented bare protein molecules carrying multiple positive charges.  These charged protein molecules are accelerated by an electric field and then deflected by a magnetic field.  They are separated according to the ratio of their mass to their charge.  The mass spectrum of a pure protein shows a set of peaks corresponding to different numbers of bound protons.  Because adjacent peaks in the mass sopectrum arise from proteins containing n-1, n, and n+1 bound protons, the mass of the parent protein molecule can be deduced.  Mass spectrometric determinations are accurate to about 1 part in 104, which means that the mass of a 1-kd protein can be measured to within 1 dalton. Vrlo niska volatility proteina je pretpostavljati prepreku za mnoge godine da koristi mass spectrometry, estazblished analitičkih tehnika u organske kemije. Poteškoća Ovo je zaobići po nedavnoj uvođenje electrospray ionization mass spectrometry. Proteina u uzorku kiselinski volatile solvent je sprayed u mass spectrometer. solvent okolnim pojedinih kapljica Evaporiti brzo u vakuum komora i instrument, ostavljajući unfragmented rodila protein molecules nošenje više pozitivnih troškove. Ovi se tereti protein molecules su ubrzane po jedna električno polje, a zatim deflected by magnetsko polje. Oni su odvojen prema omjer njihovih masa na njihove naplate. mass spectrum of čisti protein pokazuje skup vrhova koji odgovaraju različite brojeve dužni protona. Zbog susjedni vrhovi u masovnoj sopectrum proizlaze iz proteina sadrži n-1, n, i n +1 dužni protona, masa od parent protein molecule može biti deduced. Mass spectrometric determinations su točni u oko 1 dio u 104, što znači da se masa od 1-kd protein može biti mjeren u roku od 1 Dalton.