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Protein Expression Analysis - Bioinformatics Protein Expression Analysis - bioinformatiku

Analysis of Protein Expression Introduction Analiza proteina Expression Uvod

Gene expression is measured in many ways including mRNA and protein expression, however protein expression is one of the best clues of actual gene activitiy since proteins are usually final catalysts of cell activity. Ekspresija gena izmjerena je na mnoge načine, uključujući mRNA i protein expression, no expression proteina jedan je od najboljih clues stvarnih gena activitiy jer proteini su obično od završnom katalizatori stanične aktivnosti.

How can one measure protein expression? Kako se jedna mjera protein expression?

Protein expression can be analyzed using the following methods: Protein expression može biti analiziran pomoću sljedećih metoda:

Protein microarrays and high throughput (HT) mass spectrometry (MS) can provide a snapshot of the proteins present in a biological sample. Protein microarrays i visoke propusnosti (HT) masena spektrometrija (MS) mogu dati jedan snimak od proteina prisutnih u biološki uzorak. Bioinformatics is very much involved in making sense of protein microarray and HT MS data; the former approach faces similar problems as with microarrays targeted at mRNA, the latter involves the problem of matching large amounts of mass data against predicted masses from protein sequence databases, and the complicated statistical analysis of samples where multiple, but incomplete peptides from each protein are detected. Bioinformatika je vrlo mnogo koji su uključeni u stvaranje smislu protein microarray i HT MS podataka; bivše pristup lica slične probleme kao s microarrays ciljane na mRNA, potonji uključuje problem podudaranja velike količine mase podataka protiv predviđeni masa od sekvenci proteina baze podataka, i je komplicirana statistička analiza uzoraka gdje se više, ali nepotpune peptidi iz proteina svaki su otkrivena.

Bioinformatics Forum Bioinformatika Forum

Discuss and post questions about protein expression in the Bioinformatics Forum . Diskusiji i postavljati pitanja u vezi proteina izražavanja u bioinformatiku Forum.