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Hsp70I promoter in the Tol2 system

Hsp70I promoter in the Tol2 system - Zebrafish Forum

Hsp70I promoter in the Tol2 system - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 01-18-2013, 08:39 AM
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Default Hsp70I promoter in the Tol2 system



Hello,
I am currently using a Hsp70I promoter in the Tol2 system for generating transgenic zebrafish. Any suggestions for the best time and condition to do heat shock after microinjecton ? Is Hsp70I promoter leaky after recovery from heat shock? Your suggestions and comments will be highly appreciated.
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