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Morpholinos to change splicing

Morpholinos to change splicing - Zebrafish Forum

Morpholinos to change splicing - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 10-10-2012, 02:35 AM
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Default Morpholinos to change splicing



Does anyone know if splice donors or acceptors are more likely to cause inclusion of an intron? I have two morpholinos to the same exon in a gene and one is causing inclusion of the intron and the other exclusion of the exon. We would like to synthesize another MO to this gene to cause a frameshift but are unsure as to whether we should target the splice donor or acceptor?
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