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-   -   [Zbrafish] Red fluorescent reporters for transgenic fish (http://www.molecularstation.com/forum/zebrafish-forum/71315-%5Bzbrafish%5D-red-fluorescent-reporters-transgenic-fish.html)

Chi-Bin Chien 11-04-2009 06:51 PM

[Zbrafish] Red fluorescent reporters for transgenic fish
 
Dear Wei,

Very likely your problem is due to the slow maturation of DsRed.
mCherry should be better and is fairly bright. We have also had good
success with TagRFP, which is extremely bright (available commercially
from Evrogen). Another possibility is tdTomato, which is extremely
bright. We have not used it ourselves but have heard good things from
others. Don't know about maturation time.

There is an easy experiment to test whether maturation time is the
issue: do a DsRed in situ on your transgenics, and see if the
timecourse mimics the native gene.

best wishes,
Chi-Bin Chien

On Nov 2, 2009, at 5:19 PM, Wei Xia wrote:





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