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Trizol extraction - zebrafish embryos

Trizol extraction - zebrafish embryos - Zebrafish Forum

Trizol extraction - zebrafish embryos - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 11-04-2009, 06:08 PM
Kim
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Default Trizol extraction - zebrafish embryos



Has anyone encountered low 260/230 readings (<0.2) using the Trizol
RNA extraction. We're getting okay yields and great purity using 5
embryos (24hpf-72hpf)/sample, but are having problems with what we
assume is contamination.

We have already tried a number of things: bought all our chemicals
new, repeat chloroform extraction 2 times, do EtOH cleaning step 3
times. We homogenize manually with pestles.

Thanks for any advice.

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