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#1
11-04-2009, 05:08 PM
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 Trizol extraction - zebrafish embryos Has anyone encountered low 260/230 readings (<0.2) using the Trizol RNA extraction. We're getting okay yields and great purity using 5 embryos (24hpf-72hpf)/sample, but are having problems with what we assume is contamination. We have already tried a number of things: bought all our chemicals new, repeat chloroform extraction 2 times, do EtOH cleaning step 3 times. We homogenize manually with pestles. Thanks for any advice.  |
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| embryos , extraction , trizol , zebrafish |
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