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protocol needed for phospho-smad staining

protocol needed for phospho-smad staining - Zebrafish Forum

protocol needed for phospho-smad staining - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 09-30-2009, 12:10 AM
Chunyue
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Default protocol needed for phospho-smad staining



Hi All,

I tried to do immunohistochemistry using phospho-smad antibody
(purchased from Cell Signal Technology). After several trials, I
couldn't get any staining. Is anybody willing to share their
protocols?

BTW: I prefer to do the staining on whole-mount or vibrotome sections.

Thanks a lot!

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Old 03-03-2010, 05:24 PM
Pipette Filler
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Default Re: protocol needed for phospho-smad staining

hey, a u still there?

I did the same thing with zf gastrula and somite satge, it DID work well.

what do you then mean that you couldn't get anything? what stage are you looking and how is your protocol like?
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