Z Digest, Vol 50, Issue 7 fish with kinky bodies

How many days old are they? How many of the (approximately 12) fish are
affected?


Gale Clark
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+1 508 776 1468

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Sent: Friday, July 31, 2009 1:04 PM
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Subject: Zbrafish Digest, Vol 50, Issue 7

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Today's Topics:

1. Luciferase Expression Vector (Kristine Griffett)
2. Re: sick fish??? (yin alessa)


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Message: 1
Date: Thu, 30 Jul 2009 15:34:44 -0400
From: Kristine Griffett <[Only registered users see links. ].edu>
Subject: [Zbrafish] Luciferase Expression Vector
To: [Only registered users see links. ]
Message-ID: <[Only registered users see links. ].edu>
Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes

Hello All,

We would like to use a luciferase expression vector to tag cells in
zebrafish embryos and were wondering if anyone has tried to use
vectors commercially available from Promega (pRL-CMV) or New England
BioLabs (pCMV-gLUC)? Can these vectors be used "as-is" or does the
luciferase coding region need to be inserted into a Xenopus or
Zebrafish specific vector for expression of the luciferase in the
embryo?

Kristine Griffett
University of South Florida
Department of Cell Biology, Microbiology and Molecular Biology
4202 E Fowler Ave
BSF 218
Tampa, FL 33620



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Message: 2
Date: Thu, 30 Jul 2009 12:19:56 -0700 (PDT)
From: yin alessa <[Only registered users see links. ]>
Subject: [Zbrafish] Re: sick fish???
To: [Only registered users see links. ]
Message-ID:
<[Only registered users see links. ]>
Content-Type: text/plain; charset=ISO-8859-1

BTW: the fish were just changed from fry food to adult food. There
are about 6 pairs in the tank, definitely not overcrowded.



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End of Zbrafish Digest, Vol 50, Issue 7
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