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| Hello All, We would like to use a luciferase expression vector to tag cells in zebrafish embryos and were wondering if anyone has tried to use vectors commercially available from Promega (pRL-CMV) or New England BioLabs (pCMV-gLUC)? Can these vectors be used "as-is" or does the luciferase coding region need to be inserted into a Xenopus or Zebrafish specific vector for expression of the luciferase in the embryo? Kristine Griffett University of South Florida Department of Cell Biology, Microbiology and Molecular Biology 4202 E Fowler Ave BSF 218 Tampa, FL 33620 |
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| expression , luciferase , vector |
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