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Luciferase Expression Vector

Luciferase Expression Vector - Zebrafish Forum

Luciferase Expression Vector - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 07-30-2009, 07:34 PM
Kristine Griffett
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Default Luciferase Expression Vector



Hello All,

We would like to use a luciferase expression vector to tag cells in
zebrafish embryos and were wondering if anyone has tried to use
vectors commercially available from Promega (pRL-CMV) or New England
BioLabs (pCMV-gLUC)? Can these vectors be used "as-is" or does the
luciferase coding region need to be inserted into a Xenopus or
Zebrafish specific vector for expression of the luciferase in the
embryo?

Kristine Griffett
University of South Florida
Department of Cell Biology, Microbiology and Molecular Biology
4202 E Fowler Ave
BSF 218
Tampa, FL 33620

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