Fish/trout serum for zebrafish cell culture

Dear All

A collegue and I are trying to establish fish cell lines, and in
preparation for that I have found several papers strongly promoting
the addition of Fish/trout/salmonoid serum and/or embryo extracts to
the culture medium. I was wandering if anyone have experience with
using fish serum for cell culture and if so, have a tip to where I can
purchase fish serum relatively cheaply (preferably a european
company). Alternative if you have a good protochol for how to obtain
cell culture-grade fish serum from whole blood?

I would also appreciate any other comments or expertise/know how you
might like to share regarding fish cell culture, although I can be
more specific at this time as I have just started myself.

All my best, and thanks alot in advance!! Lasse

[garlic]

I know it has been a while since you have posted, but other scientists might find this of use. We are located in England, UK and we offer Seagrow (East Coast Bio) trout serum in Europe. We ship to Spain, Italy and other EU countries. If still of interest, please contact us at [Only registered users see links. ]