Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Zebrafish Forum
Register Search Today's Posts Mark Forums Read

Zebrafish Forum Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


Embryo RNA extraction using column kits

Embryo RNA extraction using column kits - Zebrafish Forum

Embryo RNA extraction using column kits - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-21-2009, 11:51 AM
Pipette Filler
Points: 300, Level: 5 Points: 300, Level: 5 Points: 300, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Unhappy Embryo RNA extraction using column kits



Hello,
We routinely use column kits (GE Illustra RNAminispin) for RNA extraction for adult tissues with good results but are now using embryos. Our RT-PCR results are generally poor compared to adult and 260/280 ratios very high (>2.2). However, reference genes look good. Is this a problem with the yolk?
From what I have read so far, most (almost all) people use Trizol in embryo work. Can someone please comment on this. Is it worth getting the Trizol?
Thanks,
Jon
Reply With Quote
Reply

Tags
column , embryo , extraction , kits , rna


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
protein purification magnificientleo Proteomics Forum 11 12-29-2010 09:01 AM
DNA plasmid extraction kits gandalfthegrey DNA Techniques 8 12-10-2010 11:32 AM
Plasmid extraction kits Jayakumar, R Protocols and Methods Forum 2 05-09-2009 08:18 AM
DNA extraction from embryo senkova@afnet.uniag.sk Protocols and Methods Forum 0 01-14-2008 10:46 AM
96-well plate DNA extraction kits Caroline Thompson Protocols and Methods Forum 2 11-13-2007 03:03 PM


All times are GMT. The time now is 08:07 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10885 seconds with 16 queries