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Morpholino dose--ng or pg per embryo

Morpholino dose--ng or pg per embryo - Zebrafish Forum

Morpholino dose--ng or pg per embryo - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 03-28-2009, 02:23 PM
Lasse
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Default Morpholino dose--ng or pg per embryo



On Mar 27, 10:31*pm, "Chao Zhang" <zhan...@ohsu.edu> wrote:

Dear Chao Zhang

I think there might be an error in your calculation... At least
according to the following way of viewing the problem, ng would be the
correct unit:

0.5mM = 5*E-4 M
1nL = 1*E-9 L
8300 g/mole = 8.3*E+3 g/mole thus

5 * E-4 * 1 * E-9 * 8.3 * E+3 = 5 * 1 * 8.3 * E-4 * E-9 * E+3 = 41,5 *
E-10 = 4,15 ng

Hope this makes sense .

All my best, and good luck with your morpholino experiments!

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