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making gels for embryo injections

making gels for embryo injections - Zebrafish Forum

making gels for embryo injections - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 02-02-2009, 08:51 AM
Marie Winandy
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Default making gels for embryo injections



Dear Jonathan,

We don't use anymore those gels, but put the eggs next to a glass microscope slide, in a dry petri dish, taking away all the water. The eggs are maintained by the slide while you injcet them. After injection you just have to rinse with a wash bottle filled with egg water and collect the eggs in a new, clean petri dish.

Good luck!

M. Winandy
Marie Winandy, PhD
Université de Liège
GIGA B34 - Zebrafish Platform
Avenue de l'Hôpital 1
B-4000 Liège - Sart Tilman
Belgique
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