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RALDH Protein Assay

RALDH Protein Assay - Zebrafish Forum

RALDH Protein Assay - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 01-30-2009, 05:22 AM
FritzXC23
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Default RALDH Protein Assay



Hi! My name is Sarah and I am undergraduate Biochemistry student at
Gettysburg College. I am currently conducting an independent research
experiment investigating the effects of vitamin A deficiency on eye
development in zebrafish. To make the embryos vitamin A deficient I am
dosing them with various concentrations of disulphiram and DEAB (two
retinaldehyde dehydrogenase inhibitors). I was wondering if anyone
could give me advice on a protein assay that I could potentially
perform that would allow me to measure RALDH activity levels in the
dosed fish. I found an article that described a BioRad assay to
measure protein concentration followed by a simple spectrophotometric
assay at 350nm. The tissue preparation described for these two assays
in regards to RALDH activity, however, was from a human source. Has
anyone performed or heard of these assays and know how to prepare
zebrafish embryos for them? Or does anyone have a better suggestion
for an assay that I could reasonably perform? Thanks for your help!

Sarah

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Old 09-03-2010, 04:05 PM
Pipette Filler
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Default Re: RALDH Protein Assay

Hi Sarah - saw your post on raldh. Did you ever get any info? There was also a bioassay mentioned in an old Brain Res Protocols paper. Could send you that if you want. AM interested in the same but for completely diff reasons, incl human cells. Can you provide more details about that BioRad assay you mentioned?
Thx
Jay
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