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making gels for embryo injections

making gels for embryo injections - Zebrafish Forum

making gels for embryo injections - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 01-29-2009, 06:42 PM
Jonathan Schumacher
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Default making gels for embryo injections



Greetings,

In "The Zebrafish Book", I see a really nice description of a device
to hold zebrafish embryos during microinjection (pg. 5.2). I'd like
to make gels using this plastic "slot maker", but don't know where to
find such a device. I've been using standard vertical wells, but it
would be nice to have 1 vertical wall to rest the embryo against & 1
sloped wall to inject from. Any idea where to purchase this "slot
maker"?

Kindest regards,

Jonathan

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  #2  
Old 01-30-2009, 02:12 PM
J.C.
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Default making gels for embryo injections

Hi, we've purchased ours from Adaptive Science tools from Worchester,
MA. Model TU-1 is the type you describe but they have a couple other
options as well.

[Only registered users see links. ]

happy injecting,
J.C.

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Old 01-30-2009, 02:39 PM
Haiqiong
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Default making gels for embryo injections

On Jan 29, 2:42*pm, Jonathan Schumacher <[Only registered users see links. ]> wrote:
Hi Jonathan,

I just use several glass microscope slides stick together with
transparent nail polish (so that it is thick enough), and tilt it in
the petri dish. It only makes one row (for holding embryos) per petri
dish. It works very well for me. Maybe you can give it a try.

Haiqiong
Fekete Lab
Purdue University




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