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in situ and engrailed staining

in situ and engrailed staining - Zebrafish Forum

in situ and engrailed staining - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 01-20-2009, 05:49 PM
Weibin Zhou
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Default in situ and engrailed staining



Hi, I am wondering whether someone would be willing to share his/her experience doing in situ and immunolabeling with monoclonal antibody 4D9 (engrailed). I have tried to do this double labeling but it appears that the antibody did not work well after the in situ procedure. I appreciate very much if someone would supply any suggestions or comments. Thank you very much.

weibin

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