about phenol red solution for microinjection

Dear Yonghua,

We use
0.5% phenol red in DPBS (Sigma, cat. no. P-0290)
This solution comes already made. I use it at 5X (1ul per 5ul total
dilution).

Best,
Wilson


Message: 2
Date: Tue, 4 Nov 2008 01:49:57 -0800 (PST)
From: Clonfish <[Only registered users see links. ]>
Subject: [Zbrafish] about phenol red solution for microinjection
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<[Only registered users see links. ]>
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Dear all,

I am trying to make a 5X stock phenol red solution for microinjection.
When I searched the literature, I found different formulas from
different papers, e.g., (0.1% phenol red, 100mM KCl) or (0.5% phenol
red, 240 mM KCl) or (1% phenol red, 200mM KCl). Do you have any idea
that which one gives the best result in your hands?

Another question is that when I prepared 1.2% phenol red solution, it
was really hard for the phenol red to be dissolved completely. Does
anybody have any suggestion for that?

Thanks a lot!

Yonghua


------------------------------------------------------------------------
--------------------
Wilson Clements, Ph.D.

[Only registered users see links. ]

Dept. of Biology
Section of Cell and Developmental Biology
University of California at San Diego
9500 Gilman Dr.
Natural Sciences Building 6105
La Jolla, CA 92093-0380

TEL (858) 534-6955
LAB (858) 822-4658
FAX (858) 822-5740

[thanhvu123]

several studies of microinjection techniques on aquatic species have been used phenol red to mix the DNA solution as a tracer is encouraged to allow confirmation of injections ; for making the injection-solution visible 0.1 % phenol-red was added; injection solutions contained 2 % phenol red to aid in estimating the volume of solution injected into each embryo.

Phenol red will be not toxic to fish embryo (abnormality) if we use it at < 0.01 %. I am doing research with 0.02% phenol red.