Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Zebrafish Forum
Register Search Today's Posts Mark Forums Read

Zebrafish Forum Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


about phenol red solution for microinjection

about phenol red solution for microinjection - Zebrafish Forum

about phenol red solution for microinjection - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-05-2008, 05:51 PM
Wilson Clements
Guest
 
Posts: n/a
Default about phenol red solution for microinjection



Dear Yonghua,

We use
0.5% phenol red in DPBS (Sigma, cat. no. P-0290)
This solution comes already made. I use it at 5X (1ul per 5ul total
dilution).

Best,
Wilson


Message: 2
Date: Tue, 4 Nov 2008 01:49:57 -0800 (PST)
From: Clonfish <[Only registered users see links. ]>
Subject: [Zbrafish] about phenol red solution for microinjection
To: [Only registered users see links. ]
Message-ID:
<[Only registered users see links. ]>
Content-Type: text/plain; charset=ISO-8859-1

Dear all,

I am trying to make a 5X stock phenol red solution for microinjection.
When I searched the literature, I found different formulas from
different papers, e.g., (0.1% phenol red, 100mM KCl) or (0.5% phenol
red, 240 mM KCl) or (1% phenol red, 200mM KCl). Do you have any idea
that which one gives the best result in your hands?

Another question is that when I prepared 1.2% phenol red solution, it
was really hard for the phenol red to be dissolved completely. Does
anybody have any suggestion for that?

Thanks a lot!

Yonghua


------------------------------------------------------------------------
--------------------
Wilson Clements, Ph.D.

[Only registered users see links. ]

Dept. of Biology
Section of Cell and Developmental Biology
University of California at San Diego
9500 Gilman Dr.
Natural Sciences Building 6105
La Jolla, CA 92093-0380

TEL (858) 534-6955
LAB (858) 822-4658
FAX (858) 822-5740



Reply With Quote
  #2  
Old 01-25-2011, 01:11 AM
Pipette Filler
Points: 67, Level: 1 Points: 67, Level: 1 Points: 67, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2011
Posts: 1
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: about phenol red solution for microinjection

several studies of microinjection techniques on aquatic species have been used phenol red to mix the DNA solution as a tracer is encouraged to allow confirmation of injections ; for making the injection-solution visible 0.1 % phenol-red was added; injection solutions contained 2 % phenol red to aid in estimating the volume of solution injected into each embryo.

Phenol red will be not toxic to fish embryo (abnormality) if we use it at < 0.01 %. I am doing research with 0.02% phenol red.
Reply With Quote
The Following User Says Thank You to thanhvu123 For This Useful Post:
admin (01-25-2011)
Reply

Tags
microinjection , phenol , red , solution


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
about phenol red solution for microinjection Clonfish Zebrafish Forum 0 11-04-2008 09:49 AM
question about phenol/water saturated solution Z.L. K Protocols and Methods Forum 2 02-11-2008 01:05 AM
Cylindrical wave, wave equation, and mistakes h_v_ansari@yahoo.com Physics Forum 2 10-26-2006 01:47 AM
question about making phenol/TE and Tim Fitzwater Protocols and Methods Forum 0 09-19-2006 05:14 PM
Sci.chem FAQ - Part 6 of 7 Bruce Hamilton Chemistry Forum 0 01-15-2004 09:12 AM


All times are GMT. The time now is 12:39 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14997 seconds with 16 queries