Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Zebrafish Forum
Register Search Today's Posts Mark Forums Read

Zebrafish Forum Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


Sense probes ISH

Sense probes ISH - Zebrafish Forum

Sense probes ISH - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-27-2008, 05:49 PM
Els
Guest
 
Posts: n/a
Default Sense probes ISH



Dear all,

I'm having difficulties to make sense probes for whole mount in situ
hybridization. There are no big differences in staining patterns
between my sense and antisense probes. Is anyone familiar with the
problem? What can I do to prevent this?

Greetz,
Els Janssens

Els Janssens
PhD-student
Research Group Neural Circuit Development and Regeneration
Department of Biology, Katholieke Universiteit Leuven (K.U.Leuven)
Naamsestraat 61, bus 2464
B-3000 Leuven, Belgium

Phone: +32 16 323987
Fax: +32 16 324262
E-mail: [Only registered users see links. ]
[Only registered users see links. ]

Reply With Quote
  #2  
Old 12-29-2008, 02:05 PM
Pipette Filler
Points: 105, Level: 2 Points: 105, Level: 2 Points: 105, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2008
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Sense probes ISH

I am facing the same problem recently. Both of my sense and antisense shown then same staining pattern.
Reply With Quote
Reply

Tags
ish , probes , sense


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Problems doing Northern Blot with small probes (oligo, riboprobe,PCR and random primed) Sharon Protocols and Methods Forum 1 02-12-2008 08:44 AM
What is Gravity? sdr@sdrodrian.com Physics Forum 1 01-27-2008 02:53 PM
Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! jon_johnfrancisayres@yahoo.com Microbiology Forum 0 10-06-2007 05:59 AM
The Achilles Heel of String Theory. S D Rodrian Physics Forum 7 07-08-2006 02:40 PM


All times are GMT. The time now is 02:39 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16712 seconds with 16 queries