Sense probes ISH

Dear all,

I'm having difficulties to make sense probes for whole mount in situ
hybridization. There are no big differences in staining patterns
between my sense and antisense probes. Is anyone familiar with the
problem? What can I do to prevent this?

Greetz,
Els Janssens

Els Janssens
PhD-student
Research Group Neural Circuit Development and Regeneration
Department of Biology, Katholieke Universiteit Leuven (K.U.Leuven)
Naamsestraat 61, bus 2464
B-3000 Leuven, Belgium

Phone: +32 16 323987
Fax: +32 16 324262
E-mail: [Only registered users see links. ]
[Only registered users see links. ]

[kahloon]

I am facing the same problem recently. Both of my sense and antisense shown then same staining pattern.