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[Zbrafish] needle calibration

[Zbrafish] needle calibration - Zebrafish Forum

[Zbrafish] needle calibration - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 08-05-2008, 05:03 PM
Jon D.Moulton
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Default [Zbrafish] needle calibration



Regarding the question:
"Hi, I just started to do microinjection. I am wondering how to
calibrate the needles so that I know the amount I am injecting."

See the supporting online material
<http://www.sciencemag.org/cgi/data/1142538/DC1/1> of the following
citation for *methods for characterizing microinjected volume* per embryo.
Riedel-Kruse IH, Muller C, Oates AC. Synchrony dynamics during
initiation, failure, and rescue of the segmentation clock.
<http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=1 7702912>
Science. 2007 Sep 28;317(5846):1911-5. Epub 2007 Aug 16.

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Best regards,

- Jon

Jon D. Moulton, Ph.D.
Diagnostics and Special Projects
GENE TOOLS, LLC
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