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| Zebrafish Forum Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens. |
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| Hi, I’m currently studying the effect of contaminants on the skin of Zebrafish. I'm trying to measure the levels of Lysozyme through the skin mucous. I tried placing 10ml of 50mM NaCl in a Ziplock bag and shaking it with the fish anaesthetized inside, but after centrifuging at 1500xg 10min 4degrees coulden't see the supernatant (fish number used were 6). So any ideas? Thank you Abdullah |
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| extraction , lysozyme , mucous , skin |
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