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Lysozyme Extraction from the skin mucous

Lysozyme Extraction from the skin mucous - Zebrafish Forum

Lysozyme Extraction from the skin mucous - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 07-23-2008, 05:57 AM
alzaidan.abdullah@gmail.com
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Default Lysozyme Extraction from the skin mucous



Hi,

Iím currently studying the effect of contaminants on the skin of
Zebrafish. I'm trying to measure the levels of Lysozyme through the
skin mucous. I tried placing 10ml of 50mM NaCl in a Ziplock bag and
shaking it with the fish anaesthetized inside, but after centrifuging
at 1500xg 10min 4degrees coulden't see the supernatant (fish number
used were 6). So any ideas?

Thank you

Abdullah

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