Lysozyme Extraction from the skin mucous
Iím currently studying the effect of contaminants on the skin of
Zebrafish. I'm trying to measure the levels of Lysozyme through the
skin mucous. I tried placing 10ml of 50mM NaCl in a Ziplock bag and
shaking it with the fish anaesthetized inside, but after centrifuging
at 1500xg 10min 4degrees coulden't see the supernatant (fish number
used were 6). So any ideas?
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