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-   -   Lysozyme Extraction from the skin mucous (http://www.molecularstation.com/forum/zebrafish-forum/21829-lysozyme-extraction-skin-mucous.html)

alzaidan.abdullah@gmail.com 07-23-2008 05:57 AM

Lysozyme Extraction from the skin mucous
 
Hi,

Iím currently studying the effect of contaminants on the skin of
Zebrafish. I'm trying to measure the levels of Lysozyme through the
skin mucous. I tried placing 10ml of 50mM NaCl in a Ziplock bag and
shaking it with the fish anaesthetized inside, but after centrifuging
at 1500xg 10min 4degrees coulden't see the supernatant (fish number
used were 6). So any ideas?

Thank you

Abdullah



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