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FW: [Zbrafish] blood extraction

FW: [Zbrafish] blood extraction - Zebrafish Forum

FW: [Zbrafish] blood extraction - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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  #1  
Old 05-29-2008, 11:00 AM
maria chiara pelleri
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Default FW: [Zbrafish] blood extraction






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Subject: RE: [Zbrafish] blood extraction
Date: Thu, 29 May 2008 09:16:34 +0000








Sorry!
I didn't specify that I need to extract the greatest quantity of blood possible because I want to extract the RNA from the sample of blood, I usually kill the fish and I use adult fish.
Can you help me?
Thanks
Maria Chiara

Maria Chiara Pelleri
University of Bologna, Italy

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Date: Wed, 28 May 2008 13:25:54 +0000
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Subject: [Zbrafish] blood extraction










Hi!
I'm searching the best method for extracting the blood from
individual zebrafish. If you had any advice or particular methods for
extracting the blood from zf, please, answer me!!!
Thanks
Maria Chiara Pelleri



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  #2  
Old 05-30-2008, 04:42 PM
Claudia
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Default FW: [Zbrafish] blood extraction

On May 29, 6:00 am, maria chiara pelleri <[Only registered users see links. ]>
wrote:

Hi Maria,
we use the method described by P. JAGADEESWARAN, J. P. SHEEHAN, F. E.
CRAIG
AND D. TROYER: Identification and characterization of zebrafish
thrombocytes. British Journal of Haematology, 1999, 107, 731-738.
Depending on the size of the zebrafish you get between 5-10ul of
blood.
Claudia

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  #3  
Old 06-03-2008, 01:23 PM
maria chiara pelleri
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Default [Zbrafish] blood extraction



Hi!
Thank you for the article.
I have an other question: to avoid that blood coagulates, where do you putthe sample after the extraction?
I tried to use the haparin, but this anticoagulant stop the polymerase activity and I need to use the sample of blood to extract RNA and make PCR...





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