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whole mount antibody staining of muscle

whole mount antibody staining of muscle - Zebrafish Forum

whole mount antibody staining of muscle - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 03-05-2008, 04:36 PM
mena22787@excite.com
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Default whole mount antibody staining of muscle



I am an undergraduate student trying to stain skeletal muscle in
embryos that are between 10-24 hpf. Thus far I've tried anti-
sarcomeric actin, zm-1, and zm-3 antibodies using a protocol that is a
modification of one found in the Zebrafish book. I have found either
very dark staining of the outside/periphery of the embryo or dark
staining of the yolk, but no specific muscle staining. We are using
the Vectastain ABC detection kit. Does anyone have any
recommendations or ideas on why we're getting this dark staining on
the outside with no inside staining? (We are using the -20C acetone
permeabilization step.)

Serena Kankash- [Only registered users see links. ]

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