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Zebrafish transgenics

Zebrafish transgenics - Zebrafish Forum

Zebrafish transgenics - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 09-25-2007, 06:18 PM
Tania Vinagre
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Default Zebrafish transgenics



I work with mice and consequently I have some limitations regarding numbers
of transgenic embryos I can obtain. For this reason, I am considering doing
some experiments in zebrafish. I am interested in getting a reasonably high
number of transgenic embryos and analyzing their skeletal phenotype. At the
moment I am not interested in producing stable lines, only embryos, but I
came across a number of possible problems. Is there any simple way to
overcome the mosaicism in zebrafish transgenics? How can I promote the
integration of my construct? Is there any good review on transgenic
techniques?

Thanks in advance

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Old 09-27-2007, 11:02 AM
Jean-Pierre Levraud
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Default Zebrafish transgenics

Hi Tania,
if what you want is simply to overexpress a protein, there is a very
simple
way to overcome mosaicism, which is not familiar to mice people:
instead of
injecting DNA, you can inject 1-cell embryos with in vitro transcribed
mRNA.
This results in ubiquitous expression and is very efficient (but
expression lasts
only for a few days).
I suggest you have a look at this paper:
Hyatt & Ekker, "Vectors and techniques for ectopic gene expression in
zebrafish",
Meth Cell Biol, 59:117 (1999)
regards,
:@)
Jean-Pierre


On Sep 25, 8:18 pm, "Tania Vinagre" <[Only registered users see links. ]> wrote:

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